Neut C, Pathak J, Romond C, Beerens H
J Assoc Off Anal Chem. 1985 Sep-Oct;68(5):881-3.
The lactose sulfite (LS) medium recommended for the detection and identification of Clostridium perfringens in foods was compared with a reference method using tryptose-sulfite-cycloserine (TSC) agar for the enumeration of this organism in a variety of foods and food ingredients. C. perfringens was detected and enumerated in 17 of the 54 samples examined with LS broth, but its presence could be confirmed in only 9 of the samples with TSC agar. In only 2 instances, C. perfringens was detected on TSC agar but not in LS broth. A positive response (FeS + and gas +) in LS broth incubated at 46 degrees C always corresponded to the presence of C. perfringens; whereas the black colonies formed on TSC agar incubated at 37 degrees C were frequently found to be Clostridium species other than C. perfringens. Thus, because of its highly selective nature, LS broth was superior to TSC agar for enumerating and confirming the small numbers of C. perfringens that were present in a majority of the samples. This was especially true when other clostridia were also present. Besides its greater selectivity and sensitivity, LS broth had the additional advantages of requiring less work and giving confirmed results within 24-48 h compared with 3 days for the TSC agar method.
将用于检测和鉴定食品中产气荚膜梭菌的乳糖亚硫酸盐（LS）培养基，与使用胰蛋白胨亚硫酸盐环丝氨酸（TSC）琼脂的参考方法进行比较，以对多种食品和食品成分中的该微生物进行计数。在用LS肉汤检测的54个样品中，有17个检测到并计数了产气荚膜梭菌，但使用TSC琼脂仅能在9个样品中确认其存在。仅在2个实例中，在TSC琼脂上检测到产气荚膜梭菌，但在LS肉汤中未检测到。在46℃孵育的LS肉汤中出现阳性反应（FeS+和气体+）总是与产气荚膜梭菌的存在相对应；而在37℃孵育的TSC琼脂上形成的黑色菌落经常被发现是除产气荚膜梭菌以外的梭菌属物种。因此，由于其高度选择性，对于大多数样品中存在的少量产气荚膜梭菌进行计数和确认时，LS肉汤优于TSC琼脂。当也存在其他梭菌时尤其如此。除了具有更高的选择性和灵敏度外，与TSC琼脂方法需要3天相比，LS肉汤还具有额外的优势，即工作量更少且能在24 - 48小时内给出确认结果。
