用烷基胺玻璃结合尿酸酶和芳基胺玻璃结合过氧化物酶对尿尿酸进行定量分析。
Quantification of urinary urate with alkylamine glass-bound uricase and arylamine glass-bound peroxidase.
作者信息
Pundir C S, Bhargava A K
机构信息
Biochemistry Research Laboratory, Department of Bio-Sciences, M. D. University, 124001 Rohtak, Haryana India.
出版信息
Indian J Clin Biochem. 2003 Jan;18(1):22-8. doi: 10.1007/BF02867661.
Uric acid in urine was analyzed using porcine liver uricase and horseradish peroxidase immobilized on alkylamine and arylamine glass beads (pore diameter 55nm) respectively. The minimum detection limit was 5.0mg/0.1 ml urine. The recovery of added uric acid was 92%. Within and between assay CVS were <1.3% and <5.3% respectively. A good correlation (r=0.93) was found between urinary urate values obtained by a commercial kit method and the present method. The uric acid in 24hr urine of apparently healthy adults and person sufering from various diseases was found to be 450 to 900mg/24hr, 659mg/24hr (range, mean) and 910-1400mg/24hr, 1145 mg/24hr (range, mean) respectively with the present method.
分别使用固定在烷基胺和芳胺玻璃珠（孔径55nm）上的猪肝尿酸酶和辣根过氧化物酶对尿液中的尿酸进行分析。最低检测限为5.0mg/0.1ml尿液。添加尿酸的回收率为92%。批内和批间变异系数分别<1.3%和<5.3%。通过商业试剂盒方法获得的尿尿酸值与本方法之间发现有良好的相关性（r=0.93）。使用本方法发现，明显健康的成年人和患有各种疾病的人的24小时尿液中的尿酸分别为450至900mg/24小时、659mg/24小时（范围，平均值）和910 - 1400mg/24小时、1145mg/24小时（范围，平均值）。
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