Latha G Suman, Lakshmi Vijaya, Rani H Surekha, Murthy K J R
10/1/1, Bhagwan Mahavir Hospital and Research Center, A.C. Guards, 500 004 Hyderabad, A.P. India.
Indian J Clin Biochem. 2005 Jul;20(2):170-3. doi: 10.1007/BF02867421.
The study was aimed at presence of specific IgE antibody levelsinvitro to the identified antigen. Based on positive skin test with Gynandropsis gynandra and elevated levels of total IgE (>325 IU/ml) 104 patients were selected. Healthy, asymptomatic individuals (25) with low total IgE (<325 IU/ml) were included as controls. The mean OD values by ELISA for specific IgE were 0.67±0.21, 0.57±0.18 and 0.56±0.18 with whole pollen antigen, 46-37 kD fraction and 36-32 kD fraction, respectively. The specificity and sensitivity between skin test positivity with whole pollen antigen verses fraction with mol.wt 46-37 kD was 90% and 90% and for fraction with mol.wt 36-32 kD was found to be 81.1% and 89.4%. The clusters with molecular weights 46-37 kD and 36-32 kD may be useful inin vitro diagnostic test. Fractions within these clusters need to be identified for a higher specificity.
该研究旨在检测体外针对已鉴定抗原的特异性IgE抗体水平。基于对白花菜的皮肤试验阳性以及总IgE水平升高(>325 IU/ml),选择了104例患者。将总IgE水平较低(<325 IU/ml)的健康无症状个体(25例)作为对照。通过ELISA法检测,针对全花粉抗原、46 - 37 kD组分和36 - 32 kD组分的特异性IgE的平均OD值分别为0.67±0.21、0.57±0.18和0.56±0.18。全花粉抗原皮肤试验阳性与分子量为46 - 37 kD的组分之间的特异性和敏感性分别为90%和90%,而分子量为36 - 32 kD的组分的特异性和敏感性分别为81.1%和89.4%。分子量为46 - 37 kD和36 - 32 kD的簇可能有助于体外诊断试验。需要鉴定这些簇内的组分以获得更高的特异性。