Akbarzadeh A, Noruzian D, Jamshidi Sh, Farhangi A, Mehrabi M R, Rad B Lame, Mofidian M, Allahverdi A
Department of Pilot Biotechnology, Pasteur Institute of Iran, 13164 Tehran, Iran.
Indian J Clin Biochem. 2007 Mar;22(1):71-6. doi: 10.1007/BF02912885.
Insulin injection is the main way to combat against insulin-dependent diabetes mellitus effects. Today in some laboratories in the world, the investigators are trying to find some treatments for this disease with insulin-secreting pancreatic islet cells transplantation. Donor tissue in each step of work was prepared from 36 adult male wistar Rats weighted 250-300 grams (75-90 days). Transplantation was done in rats after 2-4 weeks induction of diabetes with 60mg/kg of streptozotocin injection by intravenous method. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression. This technique that is called "immunoisolation" is based on the principle that transplanted tissue is protected for the host immune system by an artificial or natural membrane. In this study, the levels of insulin, C-peptide and glucose in diabetic rats have been reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells, so that testis is immunoisolated place for islet cells transplantation. Inside the testis subcutaneously and intrapretoneally implantation of pure islet cells graft, that is a natural immunoisolation method, rapidly and permanently normalized the diabetic state of streptozocin-administered animals.
胰岛素注射是对抗胰岛素依赖型糖尿病影响的主要方式。如今，世界上一些实验室的研究人员正在尝试通过移植分泌胰岛素的胰岛细胞来寻找治疗这种疾病的方法。工作的每一步中使用的供体组织均取自36只体重250 - 300克（75 - 90天）的成年雄性Wistar大鼠。通过静脉注射60mg/kg链脲佐菌素诱导糖尿病2 - 4周后，在大鼠身上进行移植。胰岛细胞的封装使得在无需免疫抑制的情况下进行移植成为可能。这种被称为“免疫隔离”的技术基于这样一个原理，即移植组织通过人工或天然膜来保护免受宿主免疫系统的攻击。在这项研究中，与未患糖尿病的大鼠相比，胰岛细胞移植20天后糖尿病大鼠的胰岛素、C肽和葡萄糖水平已恢复到正常范围，因此睾丸是胰岛细胞移植的免疫隔离部位。将纯胰岛细胞移植物皮下和腹腔内植入睾丸内，这是一种天然免疫隔离方法，能迅速且永久地使接受链脲佐菌素处理的动物的糖尿病状态恢复正常。
