College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, China.
J Agric Food Chem. 2012 Nov 21;60(46):11560-6. doi: 10.1021/jf304364c. Epub 2012 Nov 12.
In this study, a new heteropolysaccharide, here called L2, was separated from the fruit body of Lentinula edodes. Chemical and physical analyses indicated that L2 has a molecular weight of 26 KDa and consists of glucose (87.5%), galactose (9.6%), and arabinose (2.8%), but it does not possess a triple-helical conformation. Stimulation of the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in RAW 264.7 cells showed that L2 has significant immune activities involving toll-like receptor 2 (TLR2). Further studies demonstrated that the activities of L2 exhibited high stability in wide range of pH from 4.0 to 10.0, when the thermal processing temperature was below 121 °C. Our findings revealed that a new heteropolysaccharide without triple-helical conformation from Lentinula edodes shows immuno-stimulating activities involving TLR2 at modest pH and thermal processing conditions, which enable it to act as an active component in foods.
在这项研究中，从香菇子实体中分离得到一种新的杂多糖，命名为 L2。化学和物理分析表明，L2 的分子量为 26 kDa，由葡萄糖（87.5%）、半乳糖（9.6%）和阿拉伯糖（2.8%）组成，但它不具有三螺旋构象。刺激 RAW 264.7 细胞产生一氧化氮（NO）、肿瘤坏死因子 α（TNF-α）和白细胞介素 6（IL-6）表明，L2 具有涉及 Toll 样受体 2（TLR2）的显著免疫活性。进一步的研究表明，L2 的活性在 pH 值为 4.0 到 10.0 的宽范围内、当热加工温度低于 121°C 时表现出很高的稳定性。我们的研究结果表明，一种新型的、无三螺旋构象的香菇杂多糖在适度的 pH 值和热加工条件下具有 TLR2 参与的免疫刺激活性，使其能够作为食品中的活性成分发挥作用。
