College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, Guangdong 510640, People's Republic of China.
J Agric Food Chem. 2015 Jan 21;63(2):535-44. doi: 10.1021/jf504677r. Epub 2015 Jan 6.
A novel polysaccharide, here named DP1, was isolated from the fruiting body of Dictyophora indusiata using a water extraction method. Structure characterization revealed that DP1 had an average molecular weight of 1132 kDa and consisted of glucose (56.2%), galactose (14.1%), and mannose (29.7%). The main linkage type of DP1 were proven to be (1 → 3)-linked α-l-Man, (1 → 2,6)-linked α-d-Glc, (1 → 6)-linked β-d-Glc, (1 → 6)-linked β-d-Gal, and (1 → 6)-linked β-d-Man by periodate oxidation-Smith degradation and nuclear magnetic resonance analysis. The immunostimulating assay indicated that DP1 could significantly promote macrophage NO, TNF-α, and IL-6 secretion in murine RAW 264.7 cells involving complement receptor 3 (CR3). The immune activities of DP1 were quite stable under thermal processing (100, 121, and 145 °C). Besides, DP1 retained stability after acidic/alkline treatment (pH 4.0-10.0), which enabled it to be an ideal complementary medicine or functional food for therapeutics of hypoimmunity and immunodeficiency diseases.
一种新型多糖 DP1 从裂褶菌的子实体中用水提法提取得到。结构表征表明 DP1 的平均分子量为 1132 kDa，由葡萄糖（56.2%）、半乳糖（14.1%）和甘露糖（29.7%）组成。通过高碘酸氧化-Smith 降解和核磁共振分析，确定 DP1 的主要连接方式为（1→3）-连接的α-l-Man、（1→2,6）-连接的α-d-Glc、（1→6）-连接的β-d-Glc、（1→6）-连接的β-d-Gal 和（1→6）-连接的β-d-Man。免疫刺激试验表明，DP1 可以通过补体受体 3（CR3）显著促进小鼠 RAW 264.7 细胞中巨噬细胞的 NO、TNF-α 和 IL-6 分泌。DP1 的免疫活性在热加工（100、121 和 145°C）下非常稳定。此外，DP1 在酸性/碱性处理（pH 4.0-10.0）后保持稳定，这使其成为治疗低免疫和免疫缺陷疾病的理想辅助药物或功能性食品。
