中国北方人群恒牙中变形链球菌及致龋和非致龋菌的定量检测。
Quantitative detection of Streptococcus mutans and bacteria of dental caries and no caries groups in permanent teeth from a north China population.
机构信息
Laboratory Research Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
出版信息
Chin Med J (Engl). 2012 Nov;125(21):3880-4.
BACKGROUND
Streptococcus mutans (S. mutans) is the prime pathogen of dental caries. There are few reports that studied the relationship between S. mutans, bacteria and dental caries in permanent teeth when compared to those in primary teeth. This study aimed to detect S. mutans and bacteria of dental caries and non-caries groups in permanent teeth from a north China population by real-time polymerase chain reaction (PCR) and compare the relationship between the number of these bacteria and the prevalence of dental caries in permanent teeth.
METHODS
Human saliva samples were collected from 142 subjects with permanent teeth. According to their dental tooth (DT), 142 subjects were divided into a dental caries group (DT ≥ 1) and a non-caries group (DT = 0). With specific primers for S. mutans and 16S rRNA, the total number of S. mutans and total bacteria of 142 saliva samples were detected by real-time PCR and statistically analyzed.
RESULTS
There was no significant difference between the detection rates of S. mutans (P = 0.118) and medians of S. mutans (P = 0.115). The ratio of S. mutans to total bacteria in people with dental caries was significantly higher than in those without caries (P < 0.001), but the total number of bacteria in people with dental caries was significantly lower than in those without caries (P < 0.001).
CONCLUSIONS
S. mutans had different effects on caries in the permanent teeth of several individuals from a north China population. The ratios of S. mutans to total bacteria in saliva detected by real-time PCR with Sm479F/R and 16S RNA primers were closely associated with the prevalence of dental caries in the same population. These assays may be useful for the assessment of an individual's risk of dental caries.
背景
变形链球菌（S. mutans）是龋齿的主要病原体。与乳牙相比，目前鲜有研究报道恒牙中 S. mutans 与细菌和龋齿之间的关系。本研究旨在通过实时聚合酶链反应（PCR）检测中国北方人群恒牙中致龋菌和非致龋菌的 S. mutans 和细菌，并比较这些细菌数量与恒牙患龋率之间的关系。
方法
收集 142 名有恒牙的受试者的唾液样本。根据其牙齿状况（DT），将 142 名受试者分为龋齿组（DT≥1）和非龋齿组（DT=0）。采用 S. mutans 特异性引物和 16S rRNA 引物，通过实时 PCR 检测 142 份唾液样本中 S. mutans 和总细菌的总数，并进行统计学分析。
结果
S. mutans 的检出率（P=0.118）和 S. mutans 的中位数（P=0.115）之间无显著差异。患有龋齿的人群中 S. mutans 与总细菌的比值明显高于无龋齿的人群（P<0.001），而患有龋齿的人群中总细菌数量明显低于无龋齿的人群（P<0.001）。
结论
在中国北方人群的几个个体中，S. mutans 对恒牙龋齿有不同的影响。实时 PCR 检测 Sm479F/R 和 16S RNA 引物的唾液中 S. mutans 与总细菌的比值与同一人群的龋齿患病率密切相关。这些检测方法可能有助于评估个体的龋齿风险。
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