不同DNA种类对爱泼斯坦-巴尔病毒核抗原（EBNA）酸固定核结合的阻断作用。

Blocking of acid-fixed nuclear binding of Epstein-Barr virus nuclear antigen (EBNA) by different DNA species.

作者信息

Hirsch I, Kuchlerová L, Brichácek B, Suchánková A, Vonka V

出版信息

J Gen Virol. 1979 Sep;44(3):849-52. doi: 10.1099/0022-1317-44-3-849.

Abstract

Treatment of Epstein-Barr virus-determined nuclear antigen (EBNA) with DNA resulted in blocking of its ability to convert acid-fixed EBNA-negative cell nuclei to an EBNA-positive form. Epstein-Barr virus (EBV) DNA, herpes simplex virus type 2 (HSV-2) DNA and DNA isolated from three lymphoblastoid cell lines differed in their potency to block this reaction. EBV DNA was found to be about three times more effective than cellular DNAs in abolishing the ability of DNA-cellulose-purified EBNA to convert acid-fixed nuclei to the EBNA-positive form; the effect of HSV-2 DNA was of intermediate character. No difference was found between the blocking potency of DNAs isolated from EBV-genome-negative Ramos cells and EBV-genome-positive Raji and P3HR-1 cells.

摘要

用DNA处理爱泼斯坦-巴尔病毒确定的核抗原（EBNA）导致其将酸固定的EBNA阴性细胞核转化为EBNA阳性形式的能力受到阻断。爱泼斯坦-巴尔病毒（EBV）DNA、单纯疱疹病毒2型（HSV-2）DNA以及从三种淋巴母细胞系分离的DNA在阻断该反应的效力上有所不同。发现EBV DNA在消除DNA-纤维素纯化的EBNA将酸固定细胞核转化为EBNA阳性形式的能力方面比细胞DNA有效约三倍；HSV-2 DNA的作用具有中等特征。从EBV基因组阴性的拉莫斯细胞以及EBV基因组阳性的拉吉细胞和P3HR-1细胞分离的DNA的阻断效力之间未发现差异。