爱泼斯坦-巴尔病毒决定的核抗原的溶解及其作为一种DNA结合蛋白的特性鉴定。
Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.
作者信息
Luka J, Siegert W, Klein G
出版信息
J Virol. 1977 Apr;22(1):1-8. doi: 10.1128/JVI.22.1.1-8.1977.
Abstract
The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.
摘要
通过高盐处理，从两种爱泼斯坦 - 巴尔病毒（EBV）转化的细胞系——拉吉（Raji）细胞系和AW - 拉莫斯（AW - Ramos）细胞系的分离细胞核中溶解出EBV决定的核抗原（EBNA）。通过DNA - 纤维素色谱法和51Cr释放补体固定试验研究了其DNA结合特性。EBNA能与双链和单链小牛胸腺DNA结合，对双链DNA显示出更高的亲和力。从Raji细胞系和AW - Ramos细胞系制备的EBNA在DNA结合方面没有可检测到的差异。
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