Fresen K O, zur Hausen H
Proc Natl Acad Sci U S A. 1977 Jan;74(1):363-6. doi: 10.1073/pnas.74.1.363.
Infection of cells of the Epstein-Barr virus (EBV)-negative human B-lymphoma lines BJAB and Ramos with EBV preparations from P3HR-1 or B 95-8 cells converted these cells to EBV genome carriers expressing Epstein-Barr nuclear antigen (EBNA) in almost 100% of these cells. Induction of these cells as well as of clones from P3HR-1 EBV-converted BJAB cells with iododeoxyuridine, aminopterin, and hypoxanthine resulted in the appearance of a nuclear antigen in about 1-6% of the cells 1-4 days after induction. The antigen is different from known EBV-induced antigens like EBNA, viral capsid antigen (VCA) or the D- and R-subspecificities of the early antigen (EA) complex. It is demonstrated by indirect immunofluorescence and inactivated after acetone fixation. The antigen was not detectable after induction of uninfected BJAB and Ramos cells nor has it been found in noninduced or induced P3HR-1 and Raji cells. Thus, it appears that EBV-infection mediates the expression of this antigen, for which the name TINA (transiently induced nuclear antigen) is suggested. Sera reacting against TINA generally contained high antibody titers against EBV-induced EA. Only a limited number of highly EA-reactive sera, however, were also positive for TINA. Among 200 sera tested thus far, TINA reactivity was most frequently observed in sera of patients with nasopharyngeal carcinoma (7 out of 28), in sera of the only two patients with immunoblastoma tested and occasionally in sera from patients with Hodgkin's disease and chronic lymphatic leukemia. Among 70 sera from nontumor patients, TINA reactivity was observed three times: two patients suffered from "chronic" infectious mononucleosis, the other revealed persistent splenomegaly.
用来自P3HR - 1或B 95 - 8细胞的EB病毒（EBV）制剂感染EBV阴性的人B淋巴瘤细胞系BJAB和Ramos，可使这些细胞转变为EBV基因组携带者，几乎100%的这些细胞表达EB病毒核抗原（EBNA）。用碘脱氧尿苷、氨基蝶呤和次黄嘌呤诱导这些细胞以及来自P3HR - 1 EBV转化的BJAB细胞的克隆，在诱导后1 - 4天，约1 - 6%的细胞中出现一种核抗原。该抗原不同于已知的EBV诱导抗原，如EBNA、病毒衣壳抗原（VCA）或早期抗原（EA）复合物的D和R亚特异性。通过间接免疫荧光证实，且在丙酮固定后失活。未感染的BJAB和Ramos细胞诱导后未检测到该抗原，在未诱导或诱导的P3HR - 1和Raji细胞中也未发现。因此，似乎EBV感染介导了这种抗原的表达，建议将其命名为TINA（瞬时诱导核抗原）。与TINA反应的血清通常含有针对EBV诱导的EA的高抗体滴度。然而，只有有限数量的高度EA反应性血清对TINA也呈阳性。在迄今为止检测的200份血清中，TINA反应性最常见于鼻咽癌患者的血清中（28份中有7份）、仅有的两名免疫母细胞瘤患者的血清中，偶尔也见于霍奇金病和慢性淋巴细胞白血病患者的血清中。在70份非肿瘤患者的血清中，观察到3次TINA反应性：两名患者患有“慢性”传染性单核细胞增多症，另一名患者有持续性脾肿大。
