Kwon Kun-Young, Jung Hye-Ra, Hwang Ilseon, Choi Won-Il
Department of Pathology, Keimyung University School of Medicine, Daegu, Korea.
Korean J Pathol. 2012 Apr;46(2):151-61. doi: 10.4132/KoreanJPathol.2012.46.2.151. Epub 2012 Apr 25.
We investigated effects of short- and long-term exposure to sidestream smoke on the bronchiolar and alveolar cells in Sprague-Dawley rats.
RATS WERE DIVIDED INTO FIVE EXPERIMENTAL GROUPS: groups 1, 2, and 3 (1-month exposure to 3, 5, and 7 cigarettes a day, respectively), groups 4 and 5 (3- and 6 month exposure to five cigarettes a day, respectively). We examined the morphologic changes, the expressions of tumor necrosis factor α (TNF-α), tumor growth factor β1 (TGF-β1), interlekin (IL)-1α, IL-1β, Ki-67, and cytokeratin 14 and in situ apoptosis in the bronchiolar and alveolar cells on light microscopy (LM) and electron microscopic (EM) terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.
LM showed the respiratory bronchiolar dilatation and alveolar wall collapse. In groups 3, 4, and 5, EM showed loss of the cilia and Clara cells with irregular size, more prominent alveolar wall collapse and dilation of alveolar duct than those of groups 1 and 2. Bronchiolar and alveolar cells showed increased expressions of TNF-α and TGF-β in groups 4 and 5. LM and EM TUNEL stains showed increased apoptosis in groups 3, 4, and 5.
Sidestream smoke causes a bronchiolar and alveolar cell injury and the severity correlates strongly the volume and duration of exposure to sidestream smoke.
我们研究了短期和长期接触侧流烟对斯普拉格-道利大鼠细支气管和肺泡细胞的影响。
将大鼠分为五个实验组：第1、2和3组（分别每天接触3支、5支和7支香烟，为期1个月），第4和5组（分别每天接触5支香烟，为期3个月和6个月）。我们通过光学显微镜（LM）和电子显微镜（EM）的末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）染色，检查了细支气管和肺泡细胞的形态学变化、肿瘤坏死因子α（TNF-α）、肿瘤生长因子β1（TGF-β1）、白细胞介素（IL）-1α、IL-1β、Ki-67和细胞角蛋白14的表达以及原位凋亡情况。
光学显微镜显示呼吸性细支气管扩张和肺泡壁塌陷。在第3、4和5组中，电子显微镜显示纤毛和克拉拉细胞缺失，细胞大小不规则，肺泡壁塌陷和肺泡管扩张比第1和2组更明显。第4和5组的细支气管和肺泡细胞显示TNF-α和TGF-β表达增加。光学显微镜和电子显微镜TUNEL染色显示第3、4和5组凋亡增加。
侧流烟会导致细支气管和肺泡细胞损伤，其严重程度与接触侧流烟的量和持续时间密切相关。
