Department of Biology and Biochemistry, University of Bath, Bath, UK.
FEMS Yeast Res. 2013 Mar;13(2):156-61. doi: 10.1111/1567-1364.12018. Epub 2012 Nov 23.
The Saccharomyces species Saccharomyces eubayanus was recently discovered in Patagonia. Genomic, genetic and phylogenetic data all suggest that it is one of the two parents of the hybrid yeast S. pastorianus (S. cerevisiae being the other). Saccharomyces eubayanus genomes can also be found in strains of the hybrid species S. bayanus. Here, we describe a novel pair of PCR primers targeted against the S. eubayanus FSY1 gene that will specifically detect S. eubayanus and hybrids containing this allele of the gene. The primer pair can be used to identify the species using a rapid, inexpensive colony PCR method suitable for a preliminary identification of wild isolates.
最近在巴塔哥尼亚发现了酿酒酵母种的真贝酵母。基因组、遗传和系统发育数据都表明，它是杂交酵母 S. 巴氏酵母（另一个是 S. cerevisiae）的两个亲本之一。真贝酵母基因组也可以在杂交种 S. 巴氏酵母的菌株中找到。在这里，我们描述了一对针对 S. 真贝酵母 FSY1 基因的新型 PCR 引物，该引物将专门检测含有该基因等位基因的 S. 真贝酵母和杂交种。该引物对可用于使用快速、廉价的菌落 PCR 方法进行快速鉴定，该方法适用于野生分离物的初步鉴定。
