维生素C-6作为赖氨酰氧化酶辅因子作用的证据。
Evidence for the role of vitamin C-6 as a cofactor of lysyl oxidase.
作者信息
Murray J C, Levene C I
出版信息
Biochem J. 1977 Nov 1;167(2):463-7. doi: 10.1042/bj1670463.
Abstract
At 24 h after injection of 16-day chick embryos with [C-3H]pyridoxine hydrochloride, some of this label appears in the epiphysial cartilage. Over 35% of this radioactivity appears in the form of [G-3H]pyridoxal and a further 30% as other vitamin B-6 compounds. Partial purification of lysyl oxidase from the labelled epiphysial cartilage reveals a single peak of radioactivity coinciding with a single peak of enzyme activity. On dialysis against phosphate-buffered saline, 75% of this radioactivity is found to be non-diffusible. After incubation with isonicotinic acid hydrazide, a carbonyl reagent that appears to inhibit lysyl oxidase both in vivo and in vitro, a further 70% of the radioactivity is lost, with a roughly corresponding loss of enzyme activity. It is suggested that a form of vitamin B-6 is required as a cofactor of lysyl oxidase, and that this may have important implications in terms of connective-tissue metabolism.
摘要
给16日龄鸡胚注射[C-3H]盐酸吡哆醇24小时后，部分标记物出现在骺软骨中。超过35%的这种放射性以[G-3H]吡哆醛的形式出现，另外30%以其他维生素B-6化合物的形式出现。从标记的骺软骨中部分纯化赖氨酰氧化酶，发现一个放射性单峰与一个酶活性单峰重合。用磷酸盐缓冲盐水透析后，发现75%的这种放射性是不可扩散的。用异烟肼（一种似乎在体内和体外均抑制赖氨酰氧化酶的羰基试剂）孵育后，又有70%的放射性丧失，酶活性也大致相应丧失。提示维生素B-6的一种形式作为赖氨酰氧化酶的辅因子是必需的，这可能对结缔组织代谢具有重要意义。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Connective tissue defect in the chick resulting from copper deficiency.铜缺乏导致雏鸡的结缔组织缺陷。
Proc Soc Exp Biol Med. 1961 Nov;108:402-5. doi: 10.3181/00379727-108-26951.
3
PYRIDOXAL PHOSPHATE AS A CONSTITUENT OF THE HISTAMINASE (BENZYLAMINE OXIDASE) OF PIG PLASMA.磷酸吡哆醛作为猪血浆组胺酶（苄胺氧化酶）的一个组成成分
Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:45-60. doi: 10.1098/rspb.1965.0059.
4
A BIOCHEMICAL STUDY OF HUMAN SKIN COLLAGEN AND THE RELATION BETWEEN INTRA- AND INTERMOLECULAR CROSS-LINKING.人类皮肤胶原蛋白的生化研究以及分子内和分子间交联的关系
J Clin Invest. 1964 Sep;43(9):1813-23. doi: 10.1172/JCI105055.
5
Studies on copper metabolism. 32. Cardiovascular lesions in copper-deficient swine.铜代谢研究。32. 缺铜猪的心血管病变
Am J Pathol. 1962 Nov;41(5):603-21.
6
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J Exp Med. 1961 Apr 1;113(4):795-810. doi: 10.1084/jem.113.4.795.
7
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J Exp Med. 1961 Sep 1;114(3):295-310. doi: 10.1084/jem.114.3.295.
8
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Hoppe Seylers Z Physiol Chem. 1956 Dec 10;306(2-3):143-4.
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Am J Clin Nutr. 1956 Jul-Aug;4(4):318-25; discussion, 325-8. doi: 10.1093/ajcn/4.4.318.
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