Kang J O, Hudak W A, Crowley W J, Criswell B S
Medical Technology Program, University of Arizona, Tucson 85719.
Clin Chim Acta. 1990 Jan 15;186(2):285-94. doi: 10.1016/0009-8981(90)90046-u.
Alkaline phosphatase (ALP) activities were studied in peritoneal fluid obtained from patients with untreated endometriosis and control women who had normal pelves. The patients (n = 18) had significantly higher total ALP (8.65 +/- 5.69 mU/mg, p less than 0.01) and thermostable ALP (0.28 +/- 0.23 mU/mg, p less than 0.05) than the controls (n = 7) (total ALP = 2.41 +/- 1.15; thermostable ALP = 0.09 +/- 0.04 mU/mg). The contents of the thermostable ALP in the total ALP were 4.5 +/- 2.4% for controls and 3.6 +/- 2.3% for patients. To define the biochemical characteristics of the thermostable ALP, the enzyme was partially purified from a mixture of peritoneal fluid from 16 patients. The partially purified, heated ALP (PFALP) responded to the various inhibitors, activators, and pH values tested in a significantly similar manner as a purified, heated human placental ALP did. Our data suggest that PFALP may be a placental-type (or Regan) ALP.
对未经治疗的子宫内膜异位症患者以及盆腔正常的对照女性的腹腔液中的碱性磷酸酶（ALP）活性进行了研究。患者（n = 18）的总ALP（8.65 +/- 5.69 mU/mg，p < 0.01）和热稳定ALP（0.28 +/- 0.23 mU/mg，p < 0.05）显著高于对照组（n = 7）（总ALP = 2.41 +/- 1.15；热稳定ALP = 0.09 +/- 0.04 mU/mg）。对照组总ALP中热稳定ALP的含量为4.5 +/- 2.4%，患者为3.6 +/- 2.3%。为了确定热稳定ALP的生化特性，从16例患者的腹腔液混合物中对该酶进行了部分纯化。部分纯化的加热ALP（PFALP）对所测试的各种抑制剂、激活剂和pH值的反应与纯化的加热人胎盘ALP的反应显著相似。我们的数据表明，PFALP可能是胎盘型（或Regan）ALP。
