利用柠檬酸对大米中的黄曲霉毒素进行解毒
Aflatoxin Detoxification in Rice using Citric Acid.
作者信息
Safara M, Zaini F, Hashemi Sj, Mahmoudi M, Khosravi Ar, Shojai-Aliabadi F
机构信息
Science & Research Branch, Islamic Azad University (IAU), Tehran, Iran.
出版信息
Iran J Public Health. 2010;39(2):24-9. Epub 2010 Jun 30.
BACKGROUND
Aflatoxins cause health hazards to human and animals and has also economical problems. Therefore, the detoxification effect of citric acid was investigated in rice as the main food of Iranian people.
METHODS
Initially 275 samples of rice were examined for aflatoxins by HPLC. The aflatoxins contaminated samples were later treated by aqueous citric acid and detoxification of aflatoxins were quantified using HPLC.
RESULTS
Among the 275 samples analyzed, aflatoxin B(1) and aflatoxin B(2) were detected in 211(76.72% of total) samples. Aflatoxin B(1) was detected in 203(73.82% of total) samples with a mean and standard deviation of 2.3±10.21ppb. Aflatoxin B(2) together with aflatoxin B(1) were detected in only 8(2.91% of total) samples with a mean and standard deviation of 1.38±2.7ppb of aflatoxin B(2) and 2.99±1.56 of aflatoxin B(1) respectively. Aflatoxin B(1) level in 5 samples (1.82%) was above the maximum tolerated level of aflatoxin B(1) in Iran (5ppb). However considering the Iranian maximum tolerated level for aflatoxins in rice (30ppb), only 3(1.09%) samples were above the 30ppb and also in regard to the European maximum tolerated level for aflatoxins in rice (4ppb), only 9(3.27%) samples were considered as higher than 4ppb.
CONCLUSION
The HPLC assay showed that although aflatoxins with a concentration of <30 and <4 ppb in the rice samples were completely degraded, but 97.22% degradation occurred in rice contaminated with ≥30 and ≥4ppb when treated with 1N citric acid. These results revealed the efficacy of 1N citric acid in reducing aflatoxins levels in rice.
背景
黄曲霉毒素对人类和动物健康构成危害，还会引发经济问题。因此，以伊朗人主要食物大米为研究对象，考察了柠檬酸的解毒效果。
方法
最初采用高效液相色谱法（HPLC）对275份大米样本进行黄曲霉毒素检测。随后，对受黄曲霉毒素污染的样本用柠檬酸水溶液进行处理，并通过HPLC对黄曲霉毒素的解毒情况进行定量分析。
结果
在分析的275份样本中，211份（占总数的76.72%）检测出黄曲霉毒素B1和黄曲霉毒素B2。203份（占总数的73.82%）样本检测出黄曲霉毒素B1，其平均含量和标准差分别为2.3±10.21 ppb。仅8份（占总数的2.91%）样本同时检测出黄曲霉毒素B2和黄曲霉毒素B1，其中黄曲霉毒素B2的平均含量和标准差分别为1.38±2.7 ppb，黄曲霉毒素B1的平均含量和标准差分别为2.99±1.56 ppb。5份样本（1.82%）中的黄曲霉毒素B1含量高于伊朗黄曲霉毒素B1的最大耐受水平（5 ppb）。然而，考虑到伊朗大米中黄曲霉毒素的最大耐受水平为30 ppb，仅有3份（1.09%）样本超过30 ppb；就欧洲大米中黄曲霉毒素的最大耐受水平（4 ppb）而言，仅有9份（3.27%）样本被认为高于4 ppb。
结论
高效液相色谱分析表明，虽然大米样本中浓度<30 ppb和<4 ppb的黄曲霉毒素被完全降解，但用1N柠檬酸处理时，受≥30 ppb和≥4 ppb污染的大米中黄曲霉毒素降解率达97.22%。这些结果揭示了1N柠檬酸在降低大米中黄曲霉毒素水平方面的有效性。
Zotero 插件