Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.
Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.
Food Chem. 2022 Nov 30;395:133611. doi: 10.1016/j.foodchem.2022.133611. Epub 2022 Jul 1.
Rice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable results were obtained by using methanol - water (80:20, v/v) extraction followed by immunoaffinity columns for clean-up, with recoveries of 86-92%, standard deviations between 5 and 11%, LOD ranged between 0.09 and 0.32 µg/kg, and LOQ between 0.31 and 1.06 µg/kg. Method validation and sample analysis were performed by using HPLC-MS/MS. Nine rice samples from different origin, varieties and specific characteristics, acquired in Spanish supermarkets were analysed. In two basmati samples from the same batch aflatoxin B1 was detected at (1.62 ± 0.08) µg/kg and (0.77 ± 0.03) µg/kg, both lower than the levels established by European Regulation for aflatoxin B1 in cereals.
大米经常受到黄曲霉毒素的污染，这些毒素是高度有毒的真菌物质，与肝癌有很强的关联性。在这项工作中，评估了不同的提取和净化方法，以从大米中同时提取和净化黄曲霉毒素 B1、B2、G1 和 G2。采用甲醇-水（80:20，v/v）提取，然后用免疫亲和柱进行净化，得到了有利的结果，回收率为 86-92%，标准偏差在 5-11%之间，LOD 范围在 0.09-0.32µg/kg 之间，LOQ 在 0.31-1.06µg/kg 之间。采用 HPLC-MS/MS 进行方法验证和样品分析。分析了西班牙超市购买的来自不同产地、品种和特定特征的 9 个大米样品。在同一批的两个巴斯马蒂大米样品中，检测到黄曲霉毒素 B1 的含量分别为（1.62±0.08）µg/kg 和（0.77±0.03）µg/kg，均低于欧洲法规规定的谷物中黄曲霉毒素 B1 的水平。
