van Nieuwkerk E B, de Wolf C J, Kamperdijk E W, van der Baan S
Department Otorhinolaryngology, University Hospital, Amsterdam, The Netherlands.
Clin Exp Immunol. 1990 Feb;79(2):233-9. doi: 10.1111/j.1365-2249.1990.tb05184.x.
We characterized on immuno- and enzymecytochemical level the lymphoid and non-lymphoid cells in the adenoid of children with upper respiratory tract infections (URI) and otitis media with effusion (OME) and compared these with the adenoid of children with URI without OME and with the adenoid of 'healthy' children and adults. Besides macrophages and dendritic cells we also showed the presence of MHC class II positive, ciliated, epithelial cells. These non-lymphoid cells were present in all adenoids. However, their number was less than 1% of all cells. We found no difference in lymphocyte subsets from children with URI + OME compared with those from children with URI alone. These two groups showed a significant decrease of CD8-positive (suppressor/cytotoxic) cells and a slight increase in CD22-positive B cells in comparison to 'healthy' children. No difference was found in percentages of CD4-positive (helper/inducer) cells. The localization of the lymphoid subsets in adenoids of children with URI and/or OME did not differ from those of 'healthy' children and adults.
我们在免疫和酶细胞化学水平上对患有上呼吸道感染（URI）和分泌性中耳炎（OME）的儿童腺样体中的淋巴细胞和非淋巴细胞进行了表征，并将其与无OME的URI儿童的腺样体以及“健康”儿童和成人的腺样体进行了比较。除了巨噬细胞和树突状细胞外，我们还发现了MHC II类阳性的纤毛上皮细胞。这些非淋巴细胞存在于所有腺样体中。然而，它们的数量不到所有细胞的1%。我们发现，患有URI + OME的儿童与仅患有URI的儿童的淋巴细胞亚群没有差异。与“健康”儿童相比，这两组儿童的CD8阳性（抑制/细胞毒性）细胞显著减少，CD22阳性B细胞略有增加。CD4阳性（辅助/诱导）细胞的百分比没有差异。患有URI和/或OME的儿童腺样体中淋巴细胞亚群的定位与“健康”儿童和成人的没有差异。
