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1
Shigella sonnei genome sequencing and phylogenetic analysis indicate recent global dissemination from Europe.宋内志贺菌全基因组测序和系统发育分析表明其近期在全球范围内由欧洲传播而来。
Nat Genet. 2012 Sep;44(9):1056-9. doi: 10.1038/ng.2369. Epub 2012 Aug 5.
2
High resolution melt analysis to track infections due to ribotype 027 Clostridium difficile.高分辨率熔解曲线分析追踪 027 型艰难梭菌导致的感染。
J Microbiol Methods. 2012 May;89(2):87-94. doi: 10.1016/j.mimet.2012.02.011. Epub 2012 Mar 3.
3
Global Distribution of Shigella sonnei Clones.宋内志贺菌克隆株的全球分布
Emerg Infect Dis. 2011 Oct;17(10):1910-2. doi: 10.3201/eid1710.101486.
4
Development of a multiple-run high-resolution melting assay for Salmonella spp. genotyping HRM application for Salmonella spp. subtyping.用于沙门氏菌属分型的高通量多重分辨率熔解分析方法的建立
Diagn Microbiol Infect Dis. 2011 Nov;71(3):192-200. doi: 10.1016/j.diagmicrobio.2011.06.012.
5
Single nucleotide polymorphism (SNP)-based differentiation of Shigella isolates by pyrosequencing.基于焦磷酸测序的志贺氏菌分离株单核苷酸多态性（SNP）分型。
Infect Genet Evol. 2011 Oct;11(7):1761-8. doi: 10.1016/j.meegid.2011.07.015. Epub 2011 Aug 4.
6
The utility of high-resolution melting analysis of SNP nucleated PCR amplicons--an MLST based Staphylococcus aureus typing scheme.基于 SNP 核化 PCR 扩增子的高分辨率熔解分析在金黄色葡萄球菌 MLST 分型方案中的应用。
PLoS One. 2011;6(6):e19749. doi: 10.1371/journal.pone.0019749. Epub 2011 Jun 22.
7
Utility of multilocus variable-number tandem-repeat analysis as a molecular tool for phylogenetic analysis of Shigella sonnei.多位点可变数目串联重复序列分析作为宋内志贺氏菌系统发育分析分子工具的效用
J Clin Microbiol. 2009 Apr;47(4):1149-54. doi: 10.1128/JCM.01607-08. Epub 2009 Feb 18.
8
Increased isolation and characterization of Shigella sonnei obtained from hospitalized children in Tehran, Iran.从伊朗德黑兰住院儿童中分离并鉴定出更多宋内志贺菌。
J Health Popul Nutr. 2008 Dec;26(4):426-30. doi: 10.3329/jhpn.v26i4.1884.
9
Epidemiology and evolution of genotype and antimicrobial resistance of an imported Shigella sonnei clone circulating in central Taiwan.台湾中部流行的一株输入性宋内志贺菌克隆株的基因型及抗菌药物耐药性的流行病学与进化
Diagn Microbiol Infect Dis. 2007 Aug;58(4):469-75. doi: 10.1016/j.diagmicrobio.2007.03.006. Epub 2007 May 16.
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A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology.一项在六个亚洲国家开展的志贺氏菌腹泻多中心研究：疾病负担、临床表现及微生物学
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从有限的单核苷酸多态性 (SNP) 推断宋内志贺菌的全球系统发育及利用高分辨率熔解曲线分析快速、经济地对菌株进行 SNP 分型的方法。

Global phylogeny of Shigella sonnei strains from limited single nucleotide polymorphisms (SNPs) and development of a rapid and cost-effective SNP-typing scheme for strain identification by high-resolution melting analysis.

机构信息

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

出版信息

J Clin Microbiol. 2013 Jan;51(1):303-5. doi: 10.1128/JCM.02238-12. Epub 2012 Oct 31.

DOI:10.1128/JCM.02238-12
PMID:23115259
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3536230/
Abstract

The current Shigella sonnei pandemic involves geographically associated, multidrug-resistant clones. This study has demonstrated that S. sonnei phylogeny can be accurately defined with limited single nucleotide polymorphisms (SNPs). By typing 6 informative SNPs using a high-resolution melting (HRM) assay, major S. sonnei lineages/sublineages can be identified as defined by whole-genome variation.

摘要

当前的宋内志贺菌流行涉及具有地理相关性的、多药耐药克隆。本研究表明，使用有限的单核苷酸多态性（SNPs）可以准确定义宋内志贺菌的系统发育。通过使用高分辨率熔解（HRM）分析对 6 个信息性 SNPs 进行分型，可以根据全基因组变异将主要的宋内志贺菌谱系/亚谱系鉴定为定义。