Doll K, Bichler F X, Riepl H
II. Medizinischen Tierklinik der Universität München.
Dtsch Tierarztl Wochenschr. 1990 Jan;97(1):45-8.
Faecal samples of different consistency from 15 calves were subjected to two methods to separate faecal fluid. Osmolality as well as the concentrations of electrolytes and short chain fatty acids were determined for each of the fluid samples obtained and the results compared. For dialysis the following procedure was used: small dialysis bags filled with dextran 10% were placed in the cooled (4 degrees C) faecal sample to which gentamycin was added to inhibit fermentation. Equilibrium between dialysate and surrounding fluid was reached after 24 hours. However, depending on the consistency of the faeces, after 6 hours 90 to 95% of the final osmolality at 24 hours was reached. In regard to osmolality, sodium, potassium and chloride concentrations as well as to concentrations of short chain fatty acids no significant differences could be observed between the faecal fluid obtained by dialysis or by centrifugation (3500 g, 20 min, 4 degrees C) and subsequent filtration. Distinctly higher concentrations of calcium and inorganic phosphate were measured in the filtrates; the probable explanation is that these ions in faeces are largely present as poorly diffusible compounds (in particular with faeces of a more solid consistency) or bound to the cell membranes.
对15头小牛不同稠度的粪便样本采用两种方法分离粪便液。对获得的每个液体样本测定其渗透压以及电解质和短链脂肪酸的浓度，并比较结果。透析采用以下步骤：将装有10%葡聚糖的小透析袋置于冷却至4摄氏度的粪便样本中，并添加庆大霉素以抑制发酵。24小时后透析液与周围液体达到平衡。然而，根据粪便的稠度，6小时后可达到24小时最终渗透压的90%至95%。在渗透压、钠、钾和氯浓度以及短链脂肪酸浓度方面，通过透析或离心（3500g，20分钟，4摄氏度）及后续过滤获得的粪便液之间未观察到显著差异。滤液中钙和无机磷酸盐的浓度明显更高；可能的解释是，粪便中的这些离子主要以难扩散的化合物形式存在（特别是对于稠度更高的粪便）或与细胞膜结合。
