Doll K
II. Medizinischen Tierklinik, Ludwig-Maximilians-Universität München.
Tierarztl Prax. 1991 Aug;19(4):353-5.
To obtain faecal fluid from the calf by dialysis the following procedure was used: small dialysis bags filled with dextran 10% were placed in the cooled (4 degrees C) faecal sample to which gentamycin was added to inhibit fermentation. Equilibrium between dialysate and surrounding fluid was reached after 24 hours. However, depending on the consistency of the faeces, after 6 hours 90 to 95% of the final osmolality at 24 hours was reached. In regard to osmolality, sodium, potassium and chloride concentrations as well as to concentrations of short chain fatty acids no significant differences could be observed between the faecal fluid obtained by dialysis or by centrifugation (3500 g, 20 min, 4 degrees C) and subsequent filtration. Distinctly higher concentrations of calcium and inorganic phosphate were measured in the filtrates; a possible explanation is that these ions in faeces are largely present as poorly diffusible compounds (in particular with faeces of a more solid consistency) or are bound to the cell membranes.
为通过透析从小牛获取粪便液，采用了以下步骤：将装有10%葡聚糖的小透析袋置于冷却至4摄氏度的粪便样本中，并添加庆大霉素以抑制发酵。24小时后透析液与周围液体达到平衡。然而，根据粪便的稠度，6小时后可达到24小时最终渗透压的90%至95%。在渗透压、钠、钾和氯浓度以及短链脂肪酸浓度方面，通过透析或离心（3500g，20分钟，4摄氏度）并随后过滤获得的粪便液之间未观察到显著差异。滤液中测得的钙和无机磷酸盐浓度明显更高；一种可能的解释是，粪便中的这些离子主要以扩散性差的化合物形式存在（特别是对于稠度更高的粪便），或者与细胞膜结合。
