Minder H P, Merritt A M, Chalupa W
Am J Vet Res. 1980 Apr;41(4):564-7.
Feces from 13 healthy horses and 8 horses with chronic diarrhea were subjected to an in vetro fermentation procedure that had been developed for rumen fluid. Fermentations were conducted over 6 hours in a closed system, with and without an essential amino acid (EAA) mixture being added to the basic starch-buffer medium. The addition of EAA caused no significant difference in results of fermentation of feces from healthy horses. For diarrheic animals, there was a significant (P less than 0.01) increase in gas and total volatile fatty acids production whether EAA were present or not, and alpha-amino nitrogen was utilized in significantly (P less than 0.01) greater amounts only if EAA was present. Fermentations were repeated on feces from five of the eight diarrheal horses after they had been treated with oral iodochlorhydroxyquin for 1 week, and had shown desirable clinical response. A significant difference was not shown between pre- and posttreatment fermentations, except for decreased butyrate production. The results are consistent with the hypothesis that chronic equine diarrhea is primarily a colonic disease and indicates that colonic maldigestion may, in part, be responsible for excess fecal water.
对13匹健康马和8匹患有慢性腹泻的马的粪便进行了一种针对瘤胃液开发的体外发酵程序。发酵在封闭系统中进行6小时，在基本淀粉缓冲培养基中添加或不添加必需氨基酸（EAA）混合物。添加EAA对健康马粪便的发酵结果没有显著差异。对于腹泻动物，无论是否存在EAA，气体和总挥发性脂肪酸的产生均显著（P<0.01）增加，并且仅在存在EAA时，α-氨基氮的利用量才显著（P<0.01）增加。对8匹腹泻马中的5匹马经口服氯碘羟喹治疗1周并显示出良好临床反应后的粪便重复进行发酵。除丁酸盐产生减少外，治疗前和治疗后的发酵之间未显示出显著差异。结果与慢性马腹泻主要是一种结肠疾病的假设一致，并表明结肠消化不良可能部分导致粪便水分过多。
