Terpstra C, Woortmeyer R, Barteling S J
Central Veterinary Institute, Virology Department, Lelystad, The Netherlands.
Dtsch Tierarztl Wochenschr. 1990 Feb;97(2):77-9.
The Chinese strain of hog cholera virus (HCV) was adapted to suspension cultures of the established swine kidney cell line SK6. The strain designated "Cedipest", is produced on the basis of a seedlot system. The masterseed virus was identified in vitro and in vivo, and was found free from extraneous pig pathogenic viruses by repeated animal inoculation followed by appropriate serological tests. A distinct and reproducible relationship was ascertained between infectivity in vitro and protection. Pigs inoculated with 400-600 TCID50 of the Cedipest strain proved fully protected against challenge with greater than 100 pig LD50 of a virulent strain of HCV at 7 days and at 6 month post vaccination.
猪霍乱病毒（HCV）的中国毒株适应了已建立的猪肾细胞系SK6的悬浮培养。命名为“Cedipest”的毒株是基于种子批系统生产的。主种子病毒经过体外和体内鉴定，通过反复动物接种并进行适当的血清学检测，发现其不含猪源外来致病病毒。确定了体外感染性与保护作用之间存在明显且可重复的关系。接种400 - 600个组织培养感染剂量50%（TCID50）的Cedipest毒株的猪在接种疫苗后7天和6个月时，被证明对超过100个猪致死剂量50%（LD50）的HCV强毒株攻击具有完全保护作用。
