Natural Sciences and Science Education Academic Group, Nanyang Technological University, 1 Nanyang Walk, 637616, Singapore.
Anal Chem. 2012 Nov 20;84(22):10071-6. doi: 10.1021/ac302537r. Epub 2012 Nov 6.
Paper-based enzyme immobilization for a flow injection electrochemical biosensor integrated with a reagent-loaded cartridge toward a portable device was developed. A paper disk was immobilized with enzyme, then it was integrated in a flow cell as an electrochemical biosensor. A silicon tube reagent-loaded cartridge was integrated into the system, a complicated procedure was simplified as a one-click operation toward development for point-of-care applications. In this research, glucose oxidase (GOx) was employed as a model enzyme, silver ion as an inhibition reagent for GOx, and EDTA as a regeneration reagent. When GOx was inhibited by silver ions, glucose was introduced for electrochemical measurements before and after inhibited enzyme regeneration and the difference was caused by silver inhibition. The modular device has great potential for other applications, e.g., detection of enzyme activity and substrate. The platform based on double-test mode provided accurate results due to elimination of an average or control value in comparison with classical routine approaches.
基于纸张的酶固定化技术被用于开发一种与试剂加载盒集成的流动注射电化学生物传感器，以实现便携式设备。酶被固定在纸盘上，然后将其集成在流动池中作为电化学生物传感器。硅管试剂加载盒被集成到系统中，复杂的操作过程简化为一键操作，以利于开发用于即时护理应用。在这项研究中，葡萄糖氧化酶（GOx）被用作模型酶，银离子作为 GOx 的抑制试剂，EDTA 作为再生试剂。当 GOx 被银离子抑制时，在抑制酶再生前后引入葡萄糖进行电化学测量，差异是由银离子抑制引起的。基于双检测模式的模块化设备具有很大的潜力，可用于其他应用，例如检测酶活性和底物。与经典常规方法相比，该平台基于双检测模式，通过消除平均值或对照值，提供了更准确的结果。
