Boraschi D, Villa L, Volpini G, Bossù P, Censini S, Ghiara P, Scapigliat G, Nencioni L, Bartalini M, Matteucci G
Laboratory of Immunopharmacology, Sclavo Research Center, Siena, Italy.
Eur J Immunol. 1990 Feb;20(2):317-21. doi: 10.1002/eji.1830200213.
The biological activities of human recombinant interleukin (IL) 1 alpha and IL 1 beta were compared in different biological systems. The two IL 1 forms were equally active in vitro in inducing proliferation of murine thymocytes and of the murine T helper clone D10.G4.1, and in triggering release of prostaglandin E2 from human skin fibroblasts. In vivo, IL 1 alpha and IL 1 beta were similarly pyrogenic both in rabbits and mice, and could equally increase the circulating levels of the acute phase protein serum amyloid A in mice. However, only IL 1 beta showed immunostimulatory activity in vivo, as it could enhance the number of specific antibody-producing cells in the spleen of mice immunized with either a T-dependent or a T-independent antigen. Although devoid of immunostimulatory activity, IL 1 alpha could efficiently compete immunostimulation induced by IL 1 beta, suggesting an effective interaction with the IL 1 receptor. Thus, IL 1 beta appears to have an important role in the positive regulation of immune responses, while IL 1 alpha may act as down-regulator of the IL 1 beta effect.
在不同生物系统中比较了人重组白细胞介素（IL）-1α和IL-1β的生物活性。两种IL-1形式在体外诱导小鼠胸腺细胞和小鼠T辅助克隆D10.G4.1增殖以及触发人皮肤成纤维细胞释放前列腺素E2方面具有同等活性。在体内，IL-1α和IL-1β在兔子和小鼠中同样具有致热作用，并且在小鼠中同样能增加急性期蛋白血清淀粉样蛋白A的循环水平。然而，只有IL-1β在体内表现出免疫刺激活性，因为它可以增加用T依赖性或T非依赖性抗原免疫的小鼠脾脏中特异性抗体产生细胞的数量。尽管IL-1α缺乏免疫刺激活性，但它可以有效竞争IL-1β诱导的免疫刺激，提示其与IL-1受体存在有效相互作用。因此，IL-1β似乎在免疫反应的正向调节中起重要作用，而IL-1α可能作为IL-1β效应的下调因子。
