Mangenot M, Chaize J, Desfontaine M, Duvallet G, Moreau J P
Med Trop (Mars). 1979 Sep-Oct;39(5):527-30.
As the parasitological diagnosis method consisting in the detection of the parasite in people suffering from sleeping sickness did not prove entirely reliable, the authors have tested a new immunoenzymatic diagnosis method called ELISA. 41 sera from clinical suspects have been tested (parasite having been found in 16 of them). Positive and negative levels have been tested by comparison with indirect immunofluorescence. The following results, given in optical density, have been obtained: negative cases less than 36 less than or equal to uncertain cases less than or equal to 40 less than positive cases. When using these date, correlation with immunofluorescence proves to be excellent with a sensibility between 95 and 97,5 p. 100 and a specificity between 97,5 and 99 p. 100.
由于在昏睡病患者中检测寄生虫的寄生虫学诊断方法并不完全可靠，作者测试了一种名为ELISA的新型免疫酶诊断方法。对41份临床疑似患者的血清进行了检测（其中16份检测到寄生虫）。通过与间接免疫荧光法比较来检测阳性和阴性水平。得到了以下以光密度表示的结果：阴性病例小于36，小于或等于不确定病例小于或等于40，小于阳性病例。使用这些数据时，与免疫荧光法的相关性证明非常好，灵敏度在95%至97.5%之间，特异性在97.5%至99%之间。
