Zhong Yong, Wang Jun, Gu Ping, Shao Jiaqing, Lu Bin, Jiang Shisen
Department of Cardiology, School of Medicine, Nanjing University, Jinling Hospital, 305 East Zhongshan Road, Jiangsu Province, Nanjing 210002, China.
Exp Diabetes Res. 2012;2012:420854. doi: 10.1155/2012/420854. Epub 2012 Oct 17.
To investigate the effect of ezetimibe on the insulin secretion in db/db mice.
The db/db diabetic mice aged 8 weeks were randomly assigned into 2 groups and intragastrically treated with ezetimibe or placebo for 6 weeks. The age matched db/m mice served as controls. At the end of experiment, glucose tolerance test was performed and then the pancreas was collected for immunohistochemistry. In addition, in vitro perfusion of pancreatic islets was employed for the detection of insulin secretion in the first phase.
In the ezetimibe group, the fasting blood glucose was markedly reduced, and the total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were significantly lowered when compared with those in the control group (P < 0.05). At 120 min after glucose tolerance test, the area under curve in the ezetimibe group was significantly smaller than that in the control group (P < 0.05), but the AUC(INS0-30) was markedly higher. In vitro perfusion of pancreatic islets revealed the first phase insulin secretion was improved. In addition, the insulin expression in the pancreas in the ezetimibe group was significantly increased as compared to the control group.
Ezetimibe can improve glucose tolerance, recover the first phase insulin secretion, and protect the function of β cells in mice.
研究依折麦布对db/db小鼠胰岛素分泌的影响。
将8周龄的db/db糖尿病小鼠随机分为2组，分别给予依折麦布或安慰剂灌胃治疗6周。以年龄匹配的db/m小鼠作为对照。实验结束时，进行葡萄糖耐量试验，然后收集胰腺进行免疫组织化学检测。此外，采用胰岛体外灌注法检测第一相胰岛素分泌。
与对照组相比，依折麦布组空腹血糖明显降低，总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）显著下降（P<0.05）。葡萄糖耐量试验后120分钟，依折麦布组曲线下面积明显小于对照组（P<0.05），但AUC(INS0-30)明显更高。胰岛体外灌注显示第一相胰岛素分泌得到改善。此外，与对照组相比，依折麦布组胰腺中胰岛素表达明显增加。
依折麦布可改善小鼠葡萄糖耐量，恢复第一相胰岛素分泌，保护β细胞功能。
