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应用于育种计划中基因渐渗的DNA指纹图谱。

DNA fingerprints applied to gene introgression in breeding programs.

作者信息

Hillel J, Schaap T, Haberfeld A, Jeffreys A J, Plotzky Y, Cahaner A, Lavi U

机构信息

Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.

出版信息

Genetics. 1990 Mar;124(3):783-9. doi: 10.1093/genetics/124.3.783.

Abstract

An application of DNA fingerprints (DFP) for gene introgression in breeding programs of both farm animals and plants is proposed. DFP loci, detectable by minisatellite probes, are extremely polymorphic. Individuals have unique patterns of DFP and thus can be selected for maximal genomic similarity to the recipient line, and minimal similarity to the donor line, using their DFP patterns as the criterion for similarity. This genomic selection (GS) can be performed at generations BC1, BC2 or both, and thus significantly reduce the required number of backcross generations in introgression breeding programs. The association between genomic and DFP similarity is demonstrated. Theoretical distributions and variances of the relative percentages of the donor and recipient genomes as the basis for the GS approach are presented.

摘要

提出了将DNA指纹图谱(DFP)应用于家畜和植物育种计划中的基因渐渗。通过微卫星探针可检测到的DFP位点具有极高的多态性。个体具有独特的DFP模式,因此可以以其DFP模式作为相似性标准,选择与受体品系基因组相似度最高、与供体品系相似度最低的个体。这种基因组选择(GS)可以在回交一代(BC1)、回交二代(BC2)或两代同时进行,从而显著减少渐渗育种计划中所需的回交代数。证明了基因组与DFP相似性之间的关联。给出了作为GS方法基础的供体和受体基因组相对百分比的理论分布和方差。

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