Dallas J F
Department of Genetics, University of Nottingham Medical School, Queens Medical Centre, United Kingdom.
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6831-5. doi: 10.1073/pnas.85.18.6831.
A human minisatellite DNA probe detects several restriction fragment length polymorphisms in cultivars of Asian and African rice. Certain fragments appear to be inherited in a Mendelian fashion and may represent unlinked loci. The hybridization patterns appear to be cultivar-specific and largely unchanged after the regeneration of plants from tissue culture. The results suggest that these regions of the rice genome may be used to generate cultivar-specific DNA fingerprints. The demonstration of similarity between a human minisatellite sequence and polymorphic regions in the rice genome suggests that such regions also occur in the genomes of many other plant species.
一种人类小卫星DNA探针可检测亚洲和非洲水稻品种中的几种限制性片段长度多态性。某些片段似乎以孟德尔方式遗传，可能代表不连锁的基因座。杂交模式似乎具有品种特异性，并且从组织培养再生植株后基本不变。结果表明，水稻基因组的这些区域可用于生成品种特异性的DNA指纹图谱。人类小卫星序列与水稻基因组多态性区域之间相似性的证明表明，许多其他植物物种的基因组中也存在此类区域。
