Ylikoski J, Pirvola U, Närvänen O, Virtanen I
Department of Anatomy, University of Helsinki, Finland.
Hear Res. 1990 Jan;43(2-3):199-203. doi: 10.1016/0378-5955(90)90228-h.
We studied the distribution of nonerythroid spectrin, fodrin, in surface preparations and cryosections of the cochlear hair cells as well as isolated hair cells of the guinea pig by using a monoclonal antibody (Mab) reacting with Mr 240 kD alpha-fodrin polypeptide. The Mab gave a strong reaction with the cuticular plate of both the inner and outer hair cells (IHCs and OHCs). Stereocilia were nonreactive and only a weak cell surface reaction was seen in the supporting cells. In the outer hair cells the upper turns of the cochlea, fodrin was observed in a cytoplasmic spiralling structure extending from the cuticular plate towards the cell nucleus. Some labelling was also seen along the cell surface membrane and in the synaptic region. The results suggest that fodrin may be an important constituent in the active processes of hair cells such as cell motility and/or signal transduction.
我们使用一种与240kDα-血影蛋白多肽发生反应的单克隆抗体（Mab），研究了豚鼠耳蜗毛细胞的表面制剂、冷冻切片以及分离的毛细胞中非红细胞血影蛋白（肌动蛋白）的分布情况。该单克隆抗体与内毛细胞和外毛细胞（IHCs和OHCs）的表皮板发生强烈反应。静纤毛无反应，在支持细胞中仅观察到微弱的细胞表面反应。在外毛细胞中，在耳蜗上部，可观察到血影蛋白呈从表皮板向细胞核延伸的细胞质螺旋结构。在细胞表面膜和突触区域也可见到一些标记。结果表明，血影蛋白可能是毛细胞诸如细胞运动性和/或信号转导等活跃过程中的重要组成成分。
