Scarfone E, Demêmes D, Perrin D, Aunis D, Sans A
Unité INSERM U254, U.S.T.L., Montpellier, France.
Neurosci Lett. 1988 Oct 31;93(1):13-8. doi: 10.1016/0304-3940(88)90004-3.
The presence of a spectrin-related protein in rat vestibular sensory receptors was demonstrated by immunocytochemistry and immunoblotting using affinity purified anti alpha-fodrin antibodies. Intense immunoreactivity was found in the apical pole of sensory hair cells where it seems to be concentrated in the cuticular plate. In contrast, alpha-fodrin immunoreactivity was absent from the stereocilia. We suggest that a spectrin-related protein participates in the organization of the cuticular plate of vestibular hair cells by cross-linking actin filaments as well as by anchoring the cuticular plate to the apical cell membrane.
利用亲和纯化的抗α-血影蛋白抗体，通过免疫细胞化学和免疫印迹法证实了大鼠前庭感觉受体中存在一种血影蛋白相关蛋白。在感觉毛细胞的顶端极发现了强烈的免疫反应性，它似乎集中在角质板中。相比之下，静纤毛中没有α-血影蛋白免疫反应性。我们认为，一种血影蛋白相关蛋白通过交联肌动蛋白丝以及将角质板锚定到顶端细胞膜，参与了前庭毛细胞角质板的组织。
