Department of Applied Biochemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea.
J Microbiol. 2012 Oct;50(5):860-3. doi: 10.1007/s12275-012-2109-2. Epub 2012 Nov 4.
In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acid-producing strain with minimal mutation.
为了鉴定单核苷酸多态性和插入/缺失突变，我们对产赖氨酸能力增强的谷氨酸棒杆菌 ATCC 21300 菌株进行了全基因组重测序。与野生型谷氨酸棒杆菌 ATCC 13032 菌株的 3434 个预测基因相比，在 ATCC 21300 菌株中鉴定出了 142 个单核苷酸多态性和 477 个插入/缺失突变。其中，从 ATCC 21300 菌株的基因中发现了 110 个单核苷酸多态性的转换和 29 个颠换。此外，11 个参与赖氨酸生物合成途径和中心碳代谢的基因发生了单核苷酸多态性和插入/缺失突变。有趣的是，这 11 个基因的 RT-PCR 分析表明，它们在 ATCC 21300 菌株中正常表达。这些与全基因组基因相关的变异信息将有助于谷氨酸棒杆菌的基因组育种，以开发出突变最小的工业氨基酸生产菌株。
