Altwegg M, Steigerwalt A G, Altwegg-Bissig R, Lüthy-Hottenstein J, Brenner D J
Department of Medical Microbiology, University of Zurich, Switzerland.
J Clin Microbiol. 1990 Feb;28(2):258-64. doi: 10.1128/jcm.28.2.258-264.1990.
One hundred phenotypic characteristics were determined for 138 clinical and environmental Aeromonas strains. Cluster analysis revealed three major phenons equivalent to the A. hydrophila, A. caviae, and A. sobria groups, each of which contained more than one genospecies and more than one named species. An excellent correlation was found between phenotypic identification and classification based on DNA relatedness. DNA hybridization groups within each of the phenotypic groups were also separable by using a few biochemical characteristics. Key tests were production of acid from or growth on D-sorbitol (which separated DNA hybridization group 3 from groups 1 and 2 within the A. hydrophila phenogroup), growth on citrate (which essentially separated DNA hybridization group 4 from groups 5A and 5B within the A. caviae phenogroup), and growth on DL-lactate (which separated DNA hybridization group 1 from groups 2 and 3 within the A. hydrophila phenogroup as well as group 5A from groups 4 and 5B within the A. caviae phenogroup). All except one strain in the A. sobria phenogroup belonged to DNA hybridization group 8. DNA hybridization groups were not equally distributed among clinical and environmental isolates, suggesting that strains of certain DNA hybridization groups might be less virulent than others.
对138株临床和环境来源的气单胞菌菌株测定了100个表型特征。聚类分析揭示了三个主要的表型群，分别相当于嗜水气单胞菌、豚鼠气单胞菌和温和气单胞菌组，每组都包含不止一个基因种和不止一个命名种。基于DNA相关性的表型鉴定和分类之间发现了极好的相关性。每个表型组内的DNA杂交组也可以通过一些生化特征区分开来。关键试验包括从D-山梨醇产酸或在D-山梨醇上生长（将嗜水气单胞菌表型组内的DNA杂交组3与组1和组2分开）、在柠檬酸盐上生长（将豚鼠气单胞菌表型组内的DNA杂交组4与组5A和5B基本分开）以及在DL-乳酸上生长（将嗜水气单胞菌表型组内的DNA杂交组1与组2和组3分开，以及将豚鼠气单胞菌表型组内的组5A与组4和5B分开）。温和气单胞菌表型组中除一株外的所有菌株都属于DNA杂交组8。DNA杂交组在临床和环境分离株中的分布并不均匀，这表明某些DNA杂交组的菌株可能比其他菌株的毒力更低。
