Burke V, Robinson J, Atkinson H M, Gracey M
J Clin Microbiol. 1982 Jan;15(1):48-52. doi: 10.1128/jcm.15.1.48-52.1982.
Biotypes of Aeromonas spp. correlated well with enterotoxin production in a study of 174 strains. Using biochemical characteristics determined by conventional methods and multitest systems, we correctly classified 93% of the strains with regard to enterotoxin production. Most of the enterotoxigenic strains were Voges-Proskauer (VP) positive and did not hydrolyze arabinose, but VP-positive strains which hydrolyzed arabinose were mainly non-enterotoxigenic. Aeromonas punctata subsp. caviae, which is VP negative and does not oxidize gluconate or produce gas from glucose, was non-enterotoxigenic. Although the number of other VP-negative strains was small, most were enterotoxigenic. Discrimination was improved so that 97% of the strains were correctly classified if the hemolysin assay was used either for all strains or for only the VP-positive, arabinose-positive and VP-negative, non-A. punctata subsp. caviae strains. Because the proposed classification system does not require facilities for carrying out in vivo assays such as suckling mouse or ileal loop methods, the identification of enterotoxigenic Aeromonas strains should be possible in diagnostic laboratories and will facilitate epidemiological studies of the role of these organisms in acute diarrhea.
在一项对174株气单胞菌属菌株的研究中，气单胞菌属的生物型与肠毒素产生密切相关。利用传统方法和多项试验系统所确定的生化特性，我们在肠毒素产生方面对93%的菌株进行了正确分类。大多数产肠毒素菌株Voges-Proskauer（VP）试验呈阳性且不水解阿拉伯糖，但水解阿拉伯糖的VP阳性菌株主要不产肠毒素。点状气单胞菌豚鼠亚种VP试验呈阴性，不氧化葡萄糖酸盐，也不利用葡萄糖产气，不产肠毒素。虽然其他VP阴性菌株数量较少，但大多数都产肠毒素。如果对所有菌株或仅对VP阳性、阿拉伯糖阳性以及VP阴性的非点状气单胞菌豚鼠亚种菌株进行溶血素检测，分类的准确性可提高到97%。由于所提出的分类系统不需要用于进行如乳鼠或回肠袢法等体内试验的设备，因此在诊断实验室中应能够鉴定产肠毒素的气单胞菌菌株，这将有助于对这些微生物在急性腹泻中作用的流行病学研究。
