Department of Biomedical Engineering and Yonsei-Fraunhofer Medical Device Lab, Yonsei University, Wonju, Gangwon 220-710, Republic of Korea.
Med Eng Phys. 2013 Jul;35(7):1015-9. doi: 10.1016/j.medengphy.2012.10.002. Epub 2012 Nov 3.
This study tested the effectiveness of low-level laser therapy (LLLT) in preventing and/or treating osteoporotic trabecular bone. Mice were ovariectomized (OVX) to induce osteoporotic bone loss. The tibiae of eight OVX mice were treated for 5 days each week for 2 weeks by LLLT (660 nm, 3 J) using a minimally invasive laser needle system (MILNS) which is designed to minimize loss of laser energy before reaching bone (LASER group). Another eight mice received a sham treatment (SHAM group). Structural parameters of trabecular bone were measured with in vivo micro-computed tomography images before and after laser treatment. After LLLT for 2 weeks, the percentage reduction (%R) was significantly lower in BV/TV (bone volume fraction) and Tb.N (trabecular number, p<0.05 and p<0.05) and significant higher in Tb.Sp (trabecular separation) and SMI (structure model index, p<0.05 and p<0.05) than in the SHAM group. The %R in BV/TV at sites directly treated by LLLT was significantly lower in the LASER group than the SHAM group (p<0.05, p<0.05). These results indicated that LLLT using MILNS may be effective for preventing and/or treating trabecular bone loss and the effect may be site-dependent in the same bone.
本研究旨在测试低水平激光疗法（LLLT）在预防和/或治疗骨质疏松性小梁骨方面的有效性。通过卵巢切除术（OVX）诱导骨质疏松性骨丢失来建立小鼠模型。使用微创激光针系统（MILNS）每周对 8 只 OVX 小鼠的胫骨进行 5 天的治疗，共 2 周，该系统旨在最大限度地减少激光能量在到达骨骼之前的损失（激光组）。另外 8 只小鼠接受假处理（SHAM 组）。在激光治疗前后，通过体内微计算机断层扫描图像测量小梁骨的结构参数。在 LLLT 治疗 2 周后，与 SHAM 组相比，BV/TV（骨体积分数）和 Tb.N（小梁数量）的百分比降低（%R）显著降低（p<0.05 和 p<0.05），而 Tb.Sp（小梁间隔）和 SMI（结构模型指数）的%R显著增加（p<0.05 和 p<0.05）。在直接接受 LLLT 治疗的部位，BV/TV 的%R 在 LASER 组显著低于 SHAM 组（p<0.05，p<0.05）。这些结果表明，使用 MILNS 的 LLLT 可能对预防和/或治疗小梁骨丢失有效，并且在同一骨骼中，这种效果可能具有部位依赖性。
