Kolecka Anna, Chorvát Dušan, Bujdáková Helena
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynska dolina B-2, 842 15, Bratislava, Slovakia.
Folia Microbiol (Praha). 2015 Jan;60(1):45-51. doi: 10.1007/s12223-014-0338-y. Epub 2014 Aug 7.
The influence of acidic (5.6) and neutral (7.0) pH and glucose concentrations (0.9 and 2 %) was determined in in vitro biofilm formation and the cell surface hydrophobicity (CSH) in fluconazole (FLC) susceptible and tolerant yeasts of Candida albicans. The determination of biofilm viability using tetrazolium salt XTT showed that both FLC-tolerant C. albicans 1173 and FLC-sensitive C. albicans SC 5314 formed more robust biofilm in the YNB medium at pH 7.0 in the absence of FLC than that at acidic pH. Tested glucose concentrations did not show any direct effect on formation of biofilm under all conditions. However, determination of biofilm dry mass that contains also extracellular matrix suggested some effect of 2 % D-glucose. An increase in CSH (for about 10 %) was estimated in C. albicans SC 5314 in the presence of FLC, while the FLC-tolerant isolate proved a weak increase of CSH only in the YNB media containing 2 % D-glucose. Additionally, strain C. albicans SC 5314 strongly flocculated at neutral pH in the absence of FLC, but this phenomenon was not observed in the presence of FLC. Subinhibitory concentration of FLC influenced biofilm cells and CSH, but FLC susceptibility versus tolerance of C. albicans tested strains did not directly affect biofilm formation and/or CSH.
研究了酸性（5.6）和中性（7.0）pH值以及葡萄糖浓度（0.9%和2%）对白色念珠菌氟康唑（FLC）敏感和耐受菌株体外生物膜形成及细胞表面疏水性（CSH）的影响。使用四氮唑盐XTT测定生物膜活力表明，在无FLC的情况下，FLC耐受的白色念珠菌1173和FLC敏感的白色念珠菌SC 5314在pH 7.0的YNB培养基中形成的生物膜比在酸性pH条件下更坚固。在所有条件下，测试的葡萄糖浓度对生物膜形成均未显示出任何直接影响。然而，对包含细胞外基质的生物膜干重的测定表明2% D-葡萄糖有一定影响。在存在FLC的情况下，白色念珠菌SC 5314的CSH估计增加了约10%，而FLC耐受菌株仅在含有2% D-葡萄糖的YNB培养基中CSH有微弱增加。此外，在无FLC时，白色念珠菌SC 5314菌株在中性pH下强烈絮凝，但在有FLC时未观察到这种现象。FLC的亚抑菌浓度影响生物膜细胞和CSH，但白色念珠菌测试菌株对FLC的敏感性与耐受性并未直接影响生物膜形成和/或CSH。
