Nair Smita, Archer Gerald E, Tedder Thomas F
Duke University Medical Center, Durham, North Carolina.
Curr Protoc Immunol. 2012 Nov;Chapter 7:7.32.1-7.32.23. doi: 10.1002/0471142735.im0732s99.
Dendritic cells are highly specialized antigen-presenting cells (APC), which may be isolated or generated from human blood mononuclear cells. Although mature blood dendritic cells normally represent ∼0.2% of human blood mononuclear cells, their frequency can be greatly increased using the cell enrichment methods described in this unit. More highly purified dendritic cell preparations can be obtained from these populations by sorting of fluorescence-labeled cells. Alternatively, dendritic cells can be generated from monocytes by culture with the appropriate cytokines, as described here. In addition, a negative selection approach is provided that may be employed to generate cell preparations that have been depleted of dendritic cells to be used for comparison in functional studies.
树突状细胞是高度专业化的抗原呈递细胞（APC），可从人血单核细胞中分离或产生。尽管成熟的血液树突状细胞通常占人血单核细胞的约0.2%，但使用本单元所述的细胞富集方法可使其频率大幅增加。通过对荧光标记细胞进行分选，可从这些细胞群体中获得纯度更高的树突状细胞制剂。另外，如下所述，可通过用适当的细胞因子培养单核细胞来产生树突状细胞。此外，还提供了一种阴性选择方法，可用于产生已去除树突状细胞的细胞制剂，以用于功能研究中的比较。
