二维晶体图像的图像处理。

Image processing of 2D crystal images.

作者信息

Arheit Marcel, Castaño-Díez Daniel, Thierry Raphaël, Gipson Bryant R, Zeng Xiangyan, Stahlberg Henning

机构信息

C-CINA, Biozentrum, University Basel, Basel, Switzerland.

出版信息

Methods Mol Biol. 2013;955:171-94. doi: 10.1007/978-1-62703-176-9_10.

DOI:10.1007/978-1-62703-176-9_10

PMID:23132061

Abstract

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal unbending procedure to be applied to the image before evaluation in Fourier space. We here describe the process of 2D crystal image unbending, using the 2dx software system.

摘要

膜蛋白的电子晶体学利用低温透射电子显微镜对冷冻水合二维晶体进行成像。记录图像的处理利用图像中结构的周期性排列来提取傅里叶空间中衍射斑点的振幅和相位。然而，图像的缺陷需要在傅里叶空间评估之前对图像应用晶体矫正程序。我们在此描述使用2dx软件系统进行二维晶体图像矫正的过程。