电子晶体学中图像数据的合并

Merging of image data in electron crystallography.

作者信息

Arheit Marcel, Castaño-Diéz Daniel, Thierry Raphaël, Abeyrathne Priyanka, Gipson Bryant R, Stahlberg Henning

机构信息

C-CINA, Biozentrum, University Basel, Basel, Switzerland.

出版信息

Methods Mol Biol. 2013;955:195-209. doi: 10.1007/978-1-62703-176-9_11.

DOI:10.1007/978-1-62703-176-9_11

PMID:23132062

Abstract

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed image data from non-tilted and tilted 2D crystals then need to be merged into a 3D reconstruction of the membrane protein structure. We here describe the process of the 3D merging, using the 2dx software system.

摘要

膜蛋白的电子晶体学利用低温透射电子显微镜来记录冷冻水合二维晶体的图像和衍射图案。每一个二维（2D）晶体仅在一个特定倾斜角度下成像一次，并且所记录的图像可以使用2dx/MRC软件包自动处理。然后，来自非倾斜和倾斜二维晶体的处理后图像数据需要合并成膜蛋白结构的三维重建。我们在此使用2dx软件系统描述三维合并的过程。