电子晶体学中图像处理的自动化

Automation of image processing in electron crystallography.

作者信息

Arheit Marcel, Castaño-Díez Daniel, Thierry Raphaël, Gipson Bryant R, Zeng Xiangyan, Stahlberg Henning

机构信息

C-CINA, Biozentrum, University Basel, Basel, Switzerland.

出版信息

Methods Mol Biol. 2013;955:313-30. doi: 10.1007/978-1-62703-176-9_18.

DOI:10.1007/978-1-62703-176-9_18

PMID:23132069

Abstract

Electron crystallography of membrane proteins records images and diffraction patterns of frozen-hydrated two-dimensional (2D) crystals. To reconstruct the high-resolution three-dimensional (3D) structure of a membrane protein, a multitude of images of 2D crystals have to be processed. Certain processing steps are thereby similar for batches of images that were recorded under similar conditions. Here we describe how the 2dx software package can be used to automate the processing of 2D crystal images, and how the 2D and 3D merging results can be used to iteratively reprocess the images. While the processing of 2D crystal images has been fully automated, the merging process is still semi-manual.

摘要

膜蛋白的电子晶体学记录冷冻水合二维（2D）晶体的图像和衍射图案。为了重建膜蛋白的高分辨率三维（3D）结构，必须处理大量二维晶体的图像。因此，对于在相似条件下记录的一批图像，某些处理步骤是相似的。在这里，我们描述了如何使用2dx软件包自动处理二维晶体图像，以及如何使用二维和三维合并结果来迭代地重新处理图像。虽然二维晶体图像的处理已经完全自动化，但合并过程仍然是半手动的。