Mitsuoka Kaoru
Biomedicinal Information Research Center, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
Methods Mol Biol. 2013;955:539-50. doi: 10.1007/978-1-62703-176-9_29.
Electron crystallography using two-dimensional crystals of membrane protein can provide high-resolution structure of a membrane protein within a lipid bilayer. With this technique, it is advantageous to use electron diffraction patterns to collect accurate intensities of the structure factors at high resolution. Here we describe how to process diffraction patterns using the XDP program and show what parameters are used and how they are determined in the process. Finally, the procedures for merging the intensity data will be described briefly.
使用膜蛋白二维晶体的电子晶体学能够提供脂质双分子层中膜蛋白的高分辨率结构。利用这项技术,使用电子衍射图案在高分辨率下收集结构因子的准确强度是有利的。在这里,我们描述如何使用XDP程序处理衍射图案,并展示在这个过程中使用了哪些参数以及它们是如何确定的。最后,将简要描述合并强度数据的步骤。