Muto Yasuyuki, Murai Yuta, Sakihama Yasuko, Hashidoko Yasuyuki, Hashimoto Makoto
Division of Applied Science, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Sapporo 060-8589, Japan.
Biosci Biotechnol Biochem. 2012;76(11):2162-4. doi: 10.1271/bbb.120553. Epub 2012 Nov 7.
Biotin is one of the most useful tags in (bio)analytical science due to its specific interaction with avidin, but is not easy to convert because of its low solubility in most solvents. Friedel-Crafts acylation of biotin acid chloride in triflic acid was examined, and the synthesized derivatives had stronger affinity to avidin than biotin in a binding assay using 2-(4'-hydroxyphenylazo)benzoic acid.
生物素因其与抗生物素蛋白的特异性相互作用，是（生物）分析科学中最有用的标签之一，但由于其在大多数溶剂中的溶解度较低，不易进行转化。研究了在三氟甲磺酸中生物素酰氯的傅克酰基化反应，并且在使用2-（4'-羟基苯基偶氮）苯甲酸的结合试验中，合成的衍生物对抗生物素蛋白的亲和力比生物素更强。
