测定戊型肝炎病毒（HEV）SAAS-FX17 的全基因组序列，并将其作为参考，以鉴定可能的基因型 4 戊型肝炎病毒毒力决定因素。
Determination of the full-genome sequence of hepatitis E virus (HEV) SAAS-FX17 and use as a reference to identify putative HEV genotype 4 virulence determinants.
机构信息
Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.
出版信息
Virol J. 2012 Nov 8;9:264. doi: 10.1186/1743-422X-9-264.
BACKGROUND
Four major genotypes of hepatitis E virus (HEV), the causative agent of hepatitis E, have so far been recognized. While genotypes 3 and 4 are both zoonotic, the disease symptoms caused by the latter tend to be more severe. To examine if specific nucleotide/amino acid variations between genotypes 3 and 4 play a role in determining the severity of hepatitis E disease, the complete genome of one swine HEV genotype 4 isolate, SAAS-FX17, was determined and compared with other genotype 4 and genotype 3 genomes to identify putative HEV genotype 4 virulence determinants.
RESULTS
A total of 42 conformable nt/aa variations between genotype 3 and 4 HEVs were detected, of which 19 were proposed to be potential disease severity determinants for genotype 4 strains.
CONCLUSIONS
One potential determinant was located in each of the 5'-UTR and 3'-UTR, 3 and 12 within ORF1 and ORF2 respectively, and 2 in the junction region.
背景
目前已发现导致戊型肝炎的戊型肝炎病毒（HEV）有 4 种主要基因型，其中基因型 3 和 4 均为动物源性。然而，后者引起的疾病症状往往更为严重。为了研究基因型 3 和 4 之间特定的核苷酸/氨基酸变异是否在确定戊型肝炎疾病的严重程度方面发挥作用，我们确定了一株猪源 HEV 基因型 4 分离株 SAAS-FX17 的全基因组，并与其他基因型 4 和基因型 3 基因组进行了比较，以确定潜在的 HEV 基因型 4 毒力决定因素。
结果
在基因型 3 和 4 HEV 之间共检测到 42 个一致的核苷酸/氨基酸变异，其中 19 个被提议为基因型 4 株系疾病严重程度的潜在决定因素。
结论
一个潜在的决定因素分别位于 5'-UTR 和 3'-UTR、ORF1 和 ORF2 内的 3 和 12 位以及连接区。
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