Parr E L, Szary A, Parr M B
Department of Anatomy, School of Medicine, Southern Illinois University, Carbondale 62901.
J Reprod Fertil. 1990 Jan;88(1):283-94. doi: 10.1530/jrf.0.0880283.
Cells of the metrial glands of mice were isolated by enzymic or mechanical dissociation procedures. Morphological observations indicated that up to half of the enzymically dissociated cells and nearly all of the mechanically dissociated cells were granulated metrial gland cells, but the presence of some fibroblast-like stromal cells among the latter population was not ruled out. Moreover, the granulated metrial gland cells had lost a substantial part of their granule content during isolation. Both cell preparations had little or no natural killer (NK) activity, indicating either that granulated metrial gland cells are not NK-like or that their NK activity was impaired by loss of granule-associated lytic substances or by other factors. Enzymically dissociated metrial gland cells did not bind significantly to the NK target cell YAC-1, nor did they develop granules, NK activity, or the ability to bind YAC-1 cells during culture in vitro, either in normal medium or with the addition of indomethacin or lymphokines. Mechanically dissociated metrial gland cells bound avidly to YAC-1 cells but not to P815 cells or adult thymus cells, which are not NK target cells. Since many if not most of the mechanically dissociated metrial gland cells appeared morphologically to be granulated metrial gland cells, their selective binding to an NK target cell suggests that granulated metrial gland cells may be related in some way to NK cells.
通过酶解或机械解离程序分离出小鼠蜕膜细胞。形态学观察表明，高达一半的酶解细胞和几乎所有的机械解离细胞都是颗粒状蜕膜细胞，但不排除后一组细胞中存在一些成纤维细胞样基质细胞。此外，颗粒状蜕膜细胞在分离过程中失去了大部分颗粒内容物。两种细胞制剂几乎没有或没有自然杀伤（NK）活性，这表明颗粒状蜕膜细胞要么不是NK样细胞，要么其NK活性因颗粒相关溶解物质的丧失或其他因素而受损。酶解的蜕膜细胞与NK靶细胞YAC-1没有明显结合，在体外培养过程中，无论是在正常培养基中还是添加消炎痛或淋巴因子的情况下，它们都不会形成颗粒、产生NK活性或获得结合YAC-1细胞的能力。机械解离的蜕膜细胞能与YAC-1细胞强烈结合，但不与P815细胞或成年胸腺细胞结合，而P815细胞和成年胸腺细胞不是NK靶细胞。由于许多（如果不是大多数）机械解离的蜕膜细胞在形态上似乎是颗粒状蜕膜细胞，它们对NK靶细胞的选择性结合表明颗粒状蜕膜细胞可能在某种程度上与NK细胞有关。
