Bi Zhiqian, Cui Enqing, Yao Yingying, Chang Xiaoyao, Wang Xiaoyang, Zhang Yuhui, Xu Gen-Xing, Zhuang Hongqin, Hua Zi-Chun
The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, China.
Changzhou High-Tech Research Institute of Nanjing University, Changzhou, China.
Front Microbiol. 2022 Jun 30;13:927277. doi: 10.3389/fmicb.2022.927277. eCollection 2022.
has been widely administrated orally as probiotics to prevent pathogen colonization and modulate the gut microbiome balance. Endostatin is an endogenous inhibitor of angiogenesis and has been shown to inhibit tumor growth, invasion, and metastasis. At present, the combination of endostatin and chemotherapeutic drugs has been regarded as a promising antitumor treatment strategy. In this study, we selected a safe strain of as a delivery system to transport endostatin to the gastrointestinal tract and explored their combined effect on inflammatory bowel disease (IBD) and colitis-associated cancer. The results indicated that relieved dextran sulfate sodium-induced body weight loss, diarrhea, colon shortening, and epithelium damage. Long-term oral administration of significantly decreased tumor formation rate, tumor number, and tumor size. Moreover, the effect of on gut microbiota dysbiosis was also confirmed by 16S rRNA sequencing analysis. The levels of potentially beneficial bacteria, such as , , , and , were increased in the group compared to the model and groups. Meanwhile, levels of potentially pathogenic bacteria including , , and were decreased. Taken together, these results suggested that oral administration of recombinant strain may be a promising therapeutic strategy for IBD and colitis-associated cancer.
已作为益生菌被广泛口服，以预防病原体定植并调节肠道微生物群平衡。内皮抑素是一种内源性血管生成抑制剂，已被证明可抑制肿瘤生长、侵袭和转移。目前，内皮抑素与化疗药物的联合使用被认为是一种有前景的抗肿瘤治疗策略。在本研究中，我们选择了一种安全的菌株作为递送系统，将内皮抑素输送到胃肠道，并探讨它们对炎症性肠病（IBD）和结肠炎相关癌症的联合作用。结果表明，该菌株缓解了葡聚糖硫酸钠诱导的体重减轻、腹泻、结肠缩短和上皮损伤。长期口服该菌株显著降低了肿瘤形成率、肿瘤数量和肿瘤大小。此外，16S rRNA测序分析也证实了该菌株对肠道微生物群失调的影响。与模型组和对照组相比，该菌株组中潜在有益细菌如双歧杆菌、乳杆菌、阿克曼菌和粪杆菌的水平升高。同时，包括大肠杆菌、肠球菌和葡萄球菌在内的潜在致病细菌水平降低。综上所述，这些结果表明口服重组该菌株可能是治疗IBD和结肠炎相关癌症的一种有前景的治疗策略。
