载脂蛋白-1 酶活性的降低可能导致 2 型糖尿病患者 HDL 颗粒的定性损伤。
Reduction of paraoxonase-1 activity may contribute the qualitative impairment of HDL particles in patients with type 2 diabetes.
机构信息
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Zaifu-5, Hirosaki Japan.
出版信息
Diabetes Res Clin Pract. 2013 Jan;99(1):30-8. doi: 10.1016/j.diabres.2012.10.022. Epub 2012 Nov 5.
AIMS
Cholesterol efflux with high-density lipoprotein (HDL) particles has an important role in the first step of reverse cholesterol transport (RCT). However, HDL function in type 2 diabetes has not been well investigated thoroughly. We measured cholesterol efflux in 36 patients with type 2 diabetes compared with 9 controls without diabetes.
METHODS
The HDL fraction was separated with polyacrylamide gel and recovered using the protein recovery system. Concentration adjusted HDL fraction was used to determine HDL-mediated cholesterol efflux (Efflux-hdl) from THP-1 derived macrophages. We measured paraoxonase-1 (PON 1) activity to determine antioxidation capacity, serum amyloid A protein (SAA) to determine inflammatory response, and carboxymethyl-lysin (CML) to determine antiglucoxidative capacity.
RESULTS
Efflux-hdl demonstrated no correlation with plasma apoprotein A-1 (ApoA-I) or HDL-cholesterol in patients with diabetes. PON1 activity in the patients' HDL fraction was positively correlated with Efflux-hdl (r=0.39, p=0.02), and showed a negative tendency with HbA1c levels (r=-0.28, p=0.10). SAA and CML levels did not demonstrate correlation with Efflux-hdl in patients with diabetes.
CONCLUSION
We confirmed the functional changes in HDL particles in the patients. Efflux-hdl from macrophages was reduced depending upon the decrease in PON1 activity, which was inversely related to HbA1c levels.
目的
高密度脂蛋白（HDL）颗粒介导的胆固醇外排对于胆固醇逆转运（RCT）的第一步具有重要作用。然而，2 型糖尿病患者的 HDL 功能尚未得到充分研究。我们测量了 36 例 2 型糖尿病患者与 9 例无糖尿病对照者的胆固醇外排。
方法
使用聚丙烯酰胺凝胶分离 HDL 级分，并使用蛋白回收系统回收。调整浓度的 HDL 级分用于测定 THP-1 衍生巨噬细胞中 HDL 介导的胆固醇外排（Efflux-hdl）。我们测量对氧磷酶 1（PON1）活性以确定抗氧化能力，血清淀粉样蛋白 A 蛋白（SAA）以确定炎症反应，以及羧甲基赖氨酸（CML）以确定抗糖化氧化能力。
结果
糖尿病患者的 Efflux-hdl 与血浆载脂蛋白 A-1（ApoA-I）或 HDL-胆固醇无相关性。患者 HDL 级分中的 PON1 活性与 Efflux-hdl 呈正相关（r=0.39，p=0.02），并且与 HbA1c 水平呈负相关趋势（r=-0.28，p=0.10）。糖尿病患者的 SAA 和 CML 水平与 Efflux-hdl 无相关性。
结论
我们证实了患者 HDL 颗粒的功能变化。由于 PON1 活性降低，巨噬细胞的 Efflux-hdl 减少，与 HbA1c 水平呈负相关。
Zotero 插件