Department of Endocrinology, University of Groningen, The Netherlands.
Atherosclerosis. 2013 Sep;230(1):6-11. doi: 10.1016/j.atherosclerosis.2013.06.019. Epub 2013 Jul 1.
High density lipoproteins (HDL) contain paraoxonase-1 (PON-1), which has strong anti-oxidative properties. Apolipoprotein E (apoE) may enhance PON-1 activity in vitro, but the extent to which PON-1 activity is determined by circulating apoE levels is unknown. Here we determined relationships of serum PON-1 activity with apoE in subjects without and with metabolic syndrome (MetS).
We measured PON-1 activity (arylesterase activity), plasma apoE and serum amyloid A (SAA) in 93 subjects without and in 75 subjects with MetS (25 and 54 subjects with Type 2 diabetes mellitus (T2DM), respectively; p < 0.001).
PON-1 activity was lower in MetS (p < 0.005) coinciding lower HDL cholesterol, apoA-I (p < 0.001)) and SAA levels (p < 0.01), whereas apoE was increased in relation to higher triglycerides (p < 0.01). In subjects without MetS, PON-1 activity was correlated positively with apoE (r = 0.376, p < 0.001), but this relationship was absent in MetS subjects (r = 0.085, p = 0.47). Multiple linear regression analysis showed that the relationship of PON-1 activity with apoE was different in subjects with MetS compared to subjects without MetS (β = -0.270, p = 0.014 for the interaction between apoE and MetS), independently from age, sex, T2DM, use of glucose lowering drugs, anti-hypertensives and the inverse relation with SAA levels (p = 0.008). Of the individual MetS components, apoE only interacted with low HDL-C on PON-1 activity (β = -0.175, p = 0.074). The relationship of apoE with PON-1 activity was neither modified by T2DM (p = 0.49), nor by SAA (p = 0.79).
Higher apoE levels may confer higher PON-1 activity. The relationship of PON-I activity with total plasma apoE is apparently abrogated in MetS.
高密度脂蛋白（HDL）中含有对氧磷酶-1（PON-1），具有很强的抗氧化特性。载脂蛋白 E（apoE）可能在体外增强 PON-1 活性，但循环 apoE 水平对 PON-1 活性的影响程度尚不清楚。在这里，我们在无代谢综合征（MetS）和有 MetS 的受试者中分别确定了血清 PON-1 活性与 apoE 的关系。
我们测量了 93 名无 MetS 和 75 名有 MetS 的受试者（分别有 25 和 54 名患有 2 型糖尿病患者，p < 0.001）的 PON-1 活性（芳基酯酶活性）、血浆 apoE 和血清淀粉样蛋白 A（SAA）水平。
MetS 组 PON-1 活性较低（p < 0.005），同时 HDL 胆固醇、apoA-I（p < 0.001）和 SAA 水平较低（p < 0.01），而 apoE 水平与甘油三酯升高有关（p < 0.01）。在无 MetS 的受试者中，PON-1 活性与 apoE 呈正相关（r = 0.376，p < 0.001），但在 MetS 患者中这种关系不存在（r = 0.085，p = 0.47）。多元线性回归分析表明，在有 MetS 和无 MetS 的受试者中，PON-1 活性与 apoE 的关系不同（β = -0.270，p = 0.014，apoE 与 MetS 之间的交互作用），与年龄、性别、2 型糖尿病、使用降血糖药物、抗高血压药物以及与 SAA 水平的负相关（p = 0.008）无关。在单个 MetS 成分中，apoE 仅与低 HDL-C 对 PON-1 活性有相互作用（β = -0.175，p = 0.074）。apoE 与 PON-1 活性的关系不受 2 型糖尿病（p = 0.49）或 SAA（p = 0.79）的影响。
较高的 apoE 水平可能赋予较高的 PON-1 活性。在 MetS 中，PON-I 活性与总血浆 apoE 的关系显然被阻断。
