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更新白蛉属（突尼斯株）唾液腺转录组：寻找沙蝇分泌的针对人类的免疫原性蛋白。

Updating the salivary gland transcriptome of Phlebotomus papatasi (Tunisian strain): the search for sand fly-secreted immunogenic proteins for humans.

机构信息

Laboratory of Transmission, Control and Immunobiology of Infectious Diseases, Pasteur Institute of Tunis, Tunis, Tunisia.

出版信息

PLoS One. 2012;7(11):e47347. doi: 10.1371/journal.pone.0047347. Epub 2012 Nov 6.

DOI:10.1371/journal.pone.0047347
PMID:23139741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3491003/
Abstract

INTRODUCTION

Sand fly saliva plays an important role in both blood feeding and outcome of Leishmania infection. A cellular immune response against a Phlebotomus papatasi salivary protein was shown to protect rodents against Leishmania major infection. In humans, P. papatasi salivary proteins induce a systemic cellular immune response as well as a specific antisaliva humoral immune response, making these salivary proteins attractive targets as markers of exposure for this Leishmania vector. Surprisingly, the repertoire of salivary proteins reported for P. papatasi-a model sand fly for Leishmania-vector-host molecular interactions-is very limited compared with other sand fly species. We hypothesize that a more comprehensive study of the transcripts present in the salivary glands of P. papatasi will provide better knowledge of the repertoire of proteins of this important vector and will aid in selection of potential immunogenic proteins for humans and of those proteins that are highly conserved between different sand fly strains.

METHODS AND FINDINGS

A cDNA library from P. papatasi (Tunisian strain) salivary glands was constructed, and randomly selected transcripts were sequenced and analyzed. The most abundant transcripts encoding secreted proteins were identified and compared with previously reported sequences. Importantly, we identified salivary proteins not described before in this sand fly species.

CONCLUSIONS

Comparative analysis between the salivary proteins of P. papatasi from Tunisia and Israel strains shows a high level of identity, suggesting these proteins as potential common targets for markers of vector exposure or inducers of cellular immune responses in humans for different geographic areas.

摘要

简介

沙蝇唾液在吸血和利什曼原虫感染结果中都起着重要作用。针对白蛉属唾液蛋白的细胞免疫反应被证明可以保护啮齿动物免受利什曼原虫感染。在人类中，白蛉属唾液蛋白会引起全身性细胞免疫反应和特异性抗唾液体液免疫反应，这使得这些唾液蛋白成为这种利什曼原虫载体暴露的标记物的有吸引力的目标。令人惊讶的是，与其他沙蝇物种相比，报道的白蛉属唾液蛋白（利什曼原虫-媒介-宿主分子相互作用的模型沙蝇）的唾液蛋白谱非常有限。我们假设对 P. papatasi 唾液腺中存在的转录本进行更全面的研究将更好地了解该重要媒介的蛋白质谱，并有助于为人类选择潜在的免疫原性蛋白质，以及在不同沙蝇株之间高度保守的那些蛋白质。

方法和发现

构建了来自 P. papatasi（突尼斯株）唾液腺的 cDNA 文库，并对随机选择的转录本进行了测序和分析。鉴定出编码分泌蛋白的最丰富转录本，并与以前报道的序列进行了比较。重要的是，我们在这种沙蝇物种中鉴定出了以前未描述过的唾液蛋白。

结论

突尼斯和以色列菌株的 P. papatasi 唾液蛋白的比较分析显示出高度的同一性，表明这些蛋白作为潜在的共同目标，可用于标记暴露于载体的标志物或在不同地理区域诱导人类细胞免疫反应的标志物。

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