Dunn C W, Horton J W
Department of Surgery, University of Texas Southwestern Medical Center, Dallas 75235-9031.
J Surg Res. 1990 Mar;48(3):249-53. doi: 10.1016/0022-4804(90)90221-m.
Previous studies have demonstrated depression of the mononuclear phagocyte system (MPS) of which the liver comprises 80-85% in animals subjected to 21 days of obstructive jaundice. This study examined the ability of a macrophage stimulant, muramyl dipeptide (MDP), to reverse MPS dysfunction in an obstructive jaundice rat model. Sixty-two male Sprague-Dawley rats underwent sham (n = 29) laparotomy or common duct ligation (CDL) (n = 33) and were studied after 21 days. Animals were injected with 1-3.5 X 10(6) Escherichia coli via a lateral tail vein, and colony-forming units (CFU) of the liver, lung, and spleen were determined at two time intervals: 30 min postinjection to determine the phagocytic activity of MPS (sham, n = 16; CDL, n = 20) and 24 hr postinjection to determine cytotoxic activity of MPS (sham, n = 13; CDL, n = 13). MDP (3 micrograms/g) was administered subcutaneously 24 hr prior to E. coli injection in 6 sham and 10 CDL rats studied at the 30-min time interval and 7 sham and 7 CDL rats studied at the 24-hr time interval. Pretreatment with MDP appeared to reverse the impairment of phagocytic activity in the liver of CDL rats returning it to the level of sham animals (P less than 0.05). However, pretreatment with MDP did not enhance the cytotoxic activity of the MPS as evidenced by higher CFU of E. coli in the liver, lung, and spleen of CDL animals pretreated with MDP as compared to CDL animals that did not receive MDP pretreatment. This increase was only significant in the spleen.(ABSTRACT TRUNCATED AT 250 WORDS)
先前的研究表明，在经历21天阻塞性黄疸的动物中，单核吞噬细胞系统（MPS）受到抑制，其中肝脏占该系统的80 - 85%。本研究检测了巨噬细胞刺激剂胞壁酰二肽（MDP）逆转阻塞性黄疸大鼠模型中MPS功能障碍的能力。62只雄性斯普拉格 - 道利大鼠接受假手术（n = 29）或胆总管结扎（CDL）（n = 33），21天后进行研究。通过尾外侧静脉给动物注射1 - 3.5×10⁶大肠杆菌，在两个时间点测定肝脏、肺和脾脏的菌落形成单位（CFU）：注射后30分钟以测定MPS的吞噬活性（假手术组，n = 16；CDL组，n = 20），注射后24小时以测定MPS的细胞毒性活性（假手术组，n = 13；CDL组，n = 13）。在30分钟时间点研究的6只假手术大鼠和10只CDL大鼠以及24小时时间点研究的7只假手术大鼠和7只CDL大鼠中，在注射大肠杆菌前24小时皮下注射MDP（3微克/克）。MDP预处理似乎逆转了CDL大鼠肝脏中吞噬活性的损伤，使其恢复到假手术动物的水平（P < 0.05）。然而，MDP预处理并未增强MPS的细胞毒性活性，这可通过与未接受MDP预处理的CDL动物相比，接受MDP预处理的CDL动物肝脏、肺和脾脏中大肠杆菌的CFU更高得到证明。这种增加仅在脾脏中显著。（摘要截断于250字）
