Department of Urology, Tao-Yuan General Hospital, Department of Health, Taoyuan 330, Taiwan.
Life Sci. 2013 Jan 17;92(1):35-41. doi: 10.1016/j.lfs.2012.10.019. Epub 2012 Nov 7.
Studies have shown that heme oxygenase-1 (HO-1) has a protective role in the mechanism underlying hypoxic preconditioning. We used a far-infrared radiation (FIR) heater to investigate the postconditioning protective role of HO-1 against ischemia/reperfusion (I/R) injury in rat testis.
Forty rats were used. Testis ischemia was mimicked by total obstructive clamping of testis vessels for 1, 2, or 4 h, and concomitant postconditioning with 30 min FIR or heat light during initially 30 min reperfusion. HO-1 expression and apoptosis of testis tissues were examined by immunohistochemistry and in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay, respectively. HO-1 protein level and caspase-3 activity were analyzed by Western blotting.
There was less apoptotic activity in rat testis after FIR, as determined by TUNEL assay. Higher HO-1 protein expression was observed by immunohistochemistry and Western blotting (p<0.01) in testis cells after FIR postconditioning. In contrast, caspase-3 activity was significantly higher in heat light groups, as compared with FIR groups (p<0.01).
FIR postconditioning attenuated I/R injury in rat testis by inducing HO-1 expression, which might have a protective role in testis apoptosis after I/R injury.
研究表明血红素加氧酶-1（HO-1）在低氧预处理的机制中具有保护作用。我们使用远红外辐射（FIR）加热器来研究 HO-1 在大鼠睾丸缺血/再灌注（I/R）损伤中的后处理保护作用。
使用 40 只大鼠。通过完全阻塞睾丸血管来模拟睾丸缺血 1、2 或 4 小时，并在最初 30 分钟再灌注期间同时进行 30 分钟 FIR 或热光后处理。通过免疫组织化学和原位末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记（TUNEL）检测分别检测睾丸组织的 HO-1 表达和凋亡。通过 Western 印迹分析 HO-1 蛋白水平和半胱氨酸天冬氨酸蛋白酶-3（caspase-3）活性。
与热光组相比，TUNEL 检测显示 FIR 后大鼠睾丸的凋亡活性较低。免疫组织化学和 Western 印迹显示 FIR 后处理后睾丸细胞中 HO-1 蛋白表达更高（p<0.01）。相比之下，与 FIR 组相比，热光组的 caspase-3 活性明显更高（p<0.01）。
FIR 后处理通过诱导 HO-1 表达减轻了大鼠睾丸的 I/R 损伤，这可能在 I/R 损伤后睾丸细胞凋亡中具有保护作用。
