Department of Physiology (Animal Physiology II), Faculty of Biology, Universidad Complutense, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Madrid, Spain.
PLoS One. 2012;7(11):e48915. doi: 10.1371/journal.pone.0048915. Epub 2012 Nov 7.
Maternal deprivation (MD) during neonatal life has diverse long-term effects, including affectation of metabolism. Indeed, MD for 24 hours during the neonatal period reduces body weight throughout life when the animals are maintained on a normal diet. However, little information is available regarding how this early stress affects the response to increased metabolic challenges during postnatal life. We hypothesized that MD modifies the response to a high fat diet (HFD) and that this response differs between males and females. To address this question, both male and female Wistar rats were maternally deprived for 24 hours starting on the morning of postnatal day (PND) 9. Upon weaning on PND22 half of each group received a control diet (CD) and the other half HFD. MD rats of both sexes had significantly reduced accumulated food intake and weight gain compared to controls when raised on the CD. In contrast, when maintained on a HFD energy intake and weight gain did not differ between control and MD rats of either sex. However, high fat intake induced hyperleptinemia in MD rats as early as PND35, but not until PND85 in control males and control females did not become hyperleptinemic on the HFD even at PND102. High fat intake stimulated hypothalamic inflammatory markers in both male and female rats that had been exposed to MD, but not in controls. Reduced insulin sensitivity was observed only in MD males on the HFD. These results indicate that MD modifies the metabolic response to HFD intake, with this response being different between males and females. Thus, the development of obesity and secondary complications in response to high fat intake depends on numerous factors.
母体剥夺（MD）在新生儿期有多种长期影响，包括代谢的影响。事实上，在新生儿期进行 24 小时的 MD 会降低动物在正常饮食下的终生体重。然而，关于这种早期应激如何影响产后生活中对代谢挑战的反应的信息很少。我们假设 MD 会改变对高脂肪饮食（HFD）的反应，并且这种反应在雄性和雌性之间存在差异。为了解决这个问题，雄性和雌性 Wistar 大鼠从出生后第 9 天上午开始被母体剥夺 24 小时。在断奶后的第 22 天，每组一半的大鼠接受对照饮食（CD），另一半接受 HFD。与对照组相比，无论是雄性还是雌性的 MD 大鼠在接受 CD 喂养时，累积食物摄入量和体重增加明显减少。相比之下，当维持在 HFD 时，雄性和雌性 MD 大鼠的能量摄入和体重增加没有差异。然而，高脂肪摄入早在 PND35 就会导致 MD 大鼠的高瘦素血症，但在 PND85 时，对照组雄性和雌性大鼠即使在 PND102 时也不会出现高瘦素血症，而且也不会出现高瘦素血症。高脂肪摄入刺激了暴露于 MD 的雄性和雌性大鼠的下丘脑炎症标志物，但对对照组没有影响。只有在 HFD 上的 MD 雄性大鼠才观察到胰岛素敏感性降低。这些结果表明，MD 改变了对 HFD 摄入的代谢反应，这种反应在雄性和雌性之间存在差异。因此，肥胖和对高脂肪摄入的继发并发症的发展取决于许多因素。
